Intracellular temperature mapping with fluorescence-assisted photoacoustic-thermometry

Liang Gao, Chi Zhang, Chiye Li, Lihong V. Wang

Research output: Contribution to journalArticlepeer-review


Measuring intracellular temperature is critical to understanding many cellular functions but still remains challenging. Here, we present a technique-fluorescence-assisted photoacoustic thermometry (FAPT)-for intracellular temperature mapping applications. To demonstrate FAPT, we monitored the intracellular temperature distribution of HeLa cells with sub-degree (0.7 °C) temperature resolution and sub-micron (0.23 μm) spatial resolution at a sampling rate of 1 kHz. Compared to traditional fluorescence-based methods, FAPT features the unique capability of transforming a regular fluorescence probe into a concentration- and excitation-independent temperature sensor, bringing a large collection of commercially available generic fluorescent probes into the realm of intracellular temperature sensing.

Original languageEnglish (US)
Article number193705
JournalApplied Physics Letters
Issue number19
StatePublished - May 13 2013
Externally publishedYes

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)


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