Abstract
We examined changes in intranuclear chromosome positioning induced by a transcriptional activator in a simple experimental system. Targeting the VP16 acidic activation domain (AAD) to an engineered chromosome site resulted in its transcriptional activation and redistribution from a predominantly peripheral to a more interior nuclear localization. Direct visualization in vivo revealed that the chromosome site normally moves into the nuclear interior transiently in early G1 and again in early S phase. In contrast, VP16 AAD targeting induced this site's permanent interior localization in early G1. A single transcriptional activator therefore can modify the cell-cycle-dependent programme of intranuclear positioning of chromosome loci.
Original language | English (US) |
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Pages (from-to) | 134-139 |
Number of pages | 6 |
Journal | Nature Cell Biology |
Volume | 3 |
Issue number | 2 |
DOIs | |
State | Published - 2001 |
ASJC Scopus subject areas
- Cell Biology