Interphase movements of a DNA chromosome region modulated by VP16 transcriptional activator

T. Tumbar, A. S. Belmont

Research output: Contribution to journalArticlepeer-review

Abstract

We examined changes in intranuclear chromosome positioning induced by a transcriptional activator in a simple experimental system. Targeting the VP16 acidic activation domain (AAD) to an engineered chromosome site resulted in its transcriptional activation and redistribution from a predominantly peripheral to a more interior nuclear localization. Direct visualization in vivo revealed that the chromosome site normally moves into the nuclear interior transiently in early G1 and again in early S phase. In contrast, VP16 AAD targeting induced this site's permanent interior localization in early G1. A single transcriptional activator therefore can modify the cell-cycle-dependent programme of intranuclear positioning of chromosome loci.

Original languageEnglish (US)
Pages (from-to)134-139
Number of pages6
JournalNature Cell Biology
Volume3
Issue number2
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Cell Biology

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