Interferometric synthetic aperture microscopy (ISAM) is a computed imaging technique to overcome the depth-of-field limitations in optical coherence tomography/microscopy (OCT/OCM). However, the presence of optical aberrations (which typically increase with NA) can degrade the resolution of ISAM reconstructions. We demonstrate a computational adaptive optics (CAO) method to correct aberrations of a virtual (or computed) pupil. Three-dimensional datasets in rabbit muscle tissue show that ISAM with CAO astigmatism correction results in a higher resolution reconstruction than uncorrected ISAM or standard OCT/OCM. We also present our work on high-speed 2D and pseudo-3D ISAM reconstruction using a graphic processing unit (GPU). These results demonstrate ISAM with computational aberration correction in highly scattering tissue. They also demonstrate that with precise high-speed scanning, volumetric ISAM can be performed without phase noise correction, and suggest that with GPU-based processing, real-time volumetric ISAM is feasible.

Original languageEnglish (US)
Title of host publicationBiomedical Optics, BIOMED 2012
StatePublished - 2012
EventBiomedical Optics, BIOMED 2012 - Miami, FL, United States
Duration: Apr 28 2012May 2 2012


OtherBiomedical Optics, BIOMED 2012
Country/TerritoryUnited States
CityMiami, FL

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


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