Abstract

Interferometric synthetic aperture microscopy (ISAM) is a computed imaging technique to overcome the depth-of-field limitations in optical coherence tomography/microscopy (OCT/OCM). However, the presence of optical aberrations (which typically increase with NA) can degrade the resolution of ISAM reconstructions. We demonstrate a computational adaptive optics (CAO) method to correct aberrations of a virtual (or computed) pupil. Three-dimensional datasets in rabbit muscle tissue show that ISAM with CAO astigmatism correction results in a higher resolution reconstruction than uncorrected ISAM or standard OCT/OCM. We also present our work on high-speed 2D and pseudo-3D ISAM reconstruction using a graphic processing unit (GPU). These results demonstrate ISAM with computational aberration correction in highly scattering tissue. They also demonstrate that with precise high-speed scanning, volumetric ISAM can be performed without phase noise correction, and suggest that with GPU-based processing, real-time volumetric ISAM is feasible.

Original languageEnglish (US)
Title of host publicationBiomedical Optics, BIOMED 2012
StatePublished - 2012
EventBiomedical Optics, BIOMED 2012 - Miami, FL, United States
Duration: Apr 28 2012May 2 2012

Other

OtherBiomedical Optics, BIOMED 2012
CountryUnited States
CityMiami, FL
Period4/28/125/2/12

Fingerprint

synthetic apertures
adaptive optics
Microscopy
tomography
Tomography
microscopy
high resolution
scattering
aberration
high speed
Astigmatism
astigmatism
rabbits
Optical Coherence Tomography
pupils
Pupil
muscles
imaging techniques
Rabbits
Muscles

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Interferometric synthetic aperture microscopy with computational adaptive optics for high-resolution tomography of scattering tissue. / Adie, Steven G.; Ahmad, Adeel; Shemonski, Nathan; Graf, Benedikt W.; Kim, Heeseok; Hwu, Wen Mei W; Carney, P. Scott; Boppart, Stephen A.

Biomedical Optics, BIOMED 2012. 2012.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Adie, SG, Ahmad, A, Shemonski, N, Graf, BW, Kim, H, Hwu, WMW, Carney, PS & Boppart, SA 2012, Interferometric synthetic aperture microscopy with computational adaptive optics for high-resolution tomography of scattering tissue. in Biomedical Optics, BIOMED 2012. Biomedical Optics, BIOMED 2012, Miami, FL, United States, 4/28/12.
Adie SG, Ahmad A, Shemonski N, Graf BW, Kim H, Hwu WMW et al. Interferometric synthetic aperture microscopy with computational adaptive optics for high-resolution tomography of scattering tissue. In Biomedical Optics, BIOMED 2012. 2012
Adie, Steven G. ; Ahmad, Adeel ; Shemonski, Nathan ; Graf, Benedikt W. ; Kim, Heeseok ; Hwu, Wen Mei W ; Carney, P. Scott ; Boppart, Stephen A. / Interferometric synthetic aperture microscopy with computational adaptive optics for high-resolution tomography of scattering tissue. Biomedical Optics, BIOMED 2012. 2012.
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abstract = "Interferometric synthetic aperture microscopy (ISAM) is a computed imaging technique to overcome the depth-of-field limitations in optical coherence tomography/microscopy (OCT/OCM). However, the presence of optical aberrations (which typically increase with NA) can degrade the resolution of ISAM reconstructions. We demonstrate a computational adaptive optics (CAO) method to correct aberrations of a virtual (or computed) pupil. Three-dimensional datasets in rabbit muscle tissue show that ISAM with CAO astigmatism correction results in a higher resolution reconstruction than uncorrected ISAM or standard OCT/OCM. We also present our work on high-speed 2D and pseudo-3D ISAM reconstruction using a graphic processing unit (GPU). These results demonstrate ISAM with computational aberration correction in highly scattering tissue. They also demonstrate that with precise high-speed scanning, volumetric ISAM can be performed without phase noise correction, and suggest that with GPU-based processing, real-time volumetric ISAM is feasible.",
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AU - Adie, Steven G.

AU - Ahmad, Adeel

AU - Shemonski, Nathan

AU - Graf, Benedikt W.

AU - Kim, Heeseok

AU - Hwu, Wen Mei W

AU - Carney, P. Scott

AU - Boppart, Stephen A.

PY - 2012

Y1 - 2012

N2 - Interferometric synthetic aperture microscopy (ISAM) is a computed imaging technique to overcome the depth-of-field limitations in optical coherence tomography/microscopy (OCT/OCM). However, the presence of optical aberrations (which typically increase with NA) can degrade the resolution of ISAM reconstructions. We demonstrate a computational adaptive optics (CAO) method to correct aberrations of a virtual (or computed) pupil. Three-dimensional datasets in rabbit muscle tissue show that ISAM with CAO astigmatism correction results in a higher resolution reconstruction than uncorrected ISAM or standard OCT/OCM. We also present our work on high-speed 2D and pseudo-3D ISAM reconstruction using a graphic processing unit (GPU). These results demonstrate ISAM with computational aberration correction in highly scattering tissue. They also demonstrate that with precise high-speed scanning, volumetric ISAM can be performed without phase noise correction, and suggest that with GPU-based processing, real-time volumetric ISAM is feasible.

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