Intercellular transfer of mitochondria rescues virus-induced cell death but facilitates cell-to-cell spreading of porcine reproductive and respiratory syndrome virus

Rui Guo, Duane Davis, Ying Fang

Research output: Contribution to journalArticlepeer-review

Abstract

Our recent study showed that intercellular tunneling nanotubes (TNTs) serve as an alternative pathway for cell-to-cell spreading of infectious materials of porcine reproductive and respiratory syndrome virus (PRRSV). In this study, we found that PRRSV infection could induce the formation of TNTs between infected and uninfected cells. Co-culturing PRRSV-infected cells with uninfected cells, including porcine umbilical cord stem cells, rescued PRRSV-induced cell death. Mitochondrion, an important regulator of cell survival/death, was observed transferring from uninfected to PRRSV-infected cells. Importantly, impaired formation of nanotube or defective mitochondrion was unable to rescue infected cells from apoptosis/necrosis. Certain PRRSV proteins were detected to associate with mitochondria and transport from infected to uninfected cells through TNTs. Our results suggest that TNTs-transfer of functional mitochondria rescued PRRSV-infected cells from apoptosis/necrosis in the early stage of infection. On the other hand, mitochondria could be utilized as a vehicle to transport viral materials for spreading the infection.

Original languageEnglish (US)
Pages (from-to)122-134
Number of pages13
JournalVirology
Volume517
DOIs
StatePublished - Apr 2018
Externally publishedYes

Keywords

  • Cell death
  • Intercellular nanotube
  • Mitochondria
  • Porcine reproductive and respiratory syndrome virus (PRRSV)

ASJC Scopus subject areas

  • Virology

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