Interactions of poly(ethylene oxide) brushes with chemically selective surfaces

S. R. Sheth, N. Efremova, D. E. Leckband

Research output: Contribution to journalArticle

Abstract

Poly(ethylene glycol) (PEG) has long been recognized for its unusual ability to resist protein adsorption. This is attributed to the repulsion of proteins by the polymer segments. Despite its successes, there are several reports that PEG does weakly bind proteins. This work tests the hypothesis that the PEG can bind to nonpolar, hydrophobic groups such as the aliphatic side chains of amino acids. To do this we measured the forcedistance profiles between PEG5000 brushes and self-assembled alkanethiol monolayers with varying amounts of nonpolar methyl-terminal groups. The polymer adhesion to these chemically selective surfaces increased with increasing density of surface methyl groups. The equilibrium thickness of the polymer chains in contact with the alkanethiol monolayer decreased correspondingly. The brush did not adhere to lipid bilayers or to bare mica. The results show that PEG will adsorb to nonpolar, hydrophobic surfaces. These findings may provide a possible explanation for previous direct force measurements of protein-PEG adhesion, and reports of PEG complexation with partially folded proteins.

Original languageEnglish (US)
Pages (from-to)7652-7662
Number of pages11
JournalJournal of Physical Chemistry B
Volume104
Issue number32
StatePublished - Aug 17 2000

Fingerprint

Ethylene Oxide
selective surfaces
brushes
Brushes
ethylene oxide
Polyethylene oxides
Polyethylene glycols
proteins
Polymers
Proteins
interactions
polymers
adhesion
Ethylene Glycol
Adhesion
Lipid Bilayers
mica
Adsorption
amino acids
lipids

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Interactions of poly(ethylene oxide) brushes with chemically selective surfaces. / Sheth, S. R.; Efremova, N.; Leckband, D. E.

In: Journal of Physical Chemistry B, Vol. 104, No. 32, 17.08.2000, p. 7652-7662.

Research output: Contribution to journalArticle

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