Interactions between Nucleotide Binding Sites on Chloroplast Coupling Factor 1 during ATP Hydrolysis

Deborah E Leckband, Gordon G. Hammes

Research output: Contribution to journalArticle

Abstract

The initial hydrolysis of CaATP by chloroplast coupling factor 1 was studied with the quenched-flow method. The time course of hydrolysis can be described as a first-order conversion of the enzyme to an active form followed by steady-state formation of product. The rate constant for the first-order process is independent of substrate concentration but increases hyperbolically to a limiting value of 0.43 s-1 with increasing concentrations of free Ca2+. A mechanism involving a Ca2+-triggered conversion to an active form of the enzyme is consistent with the data. The steady-state rate varied sigmoidally with the CaATP concentration. Initial exchange of tightly bound ADP is complex: ∼50% of the bound nucleotide is lost within 30 s, with complete exchange requiring several minutes. The first-order rate constant characterizing the rapid phase of the reaction increases hyperbolically to a limiting value of 0.26 s-1 as the concentration of CaATP is increased, indicating that the binding of CaATP to the enzyme promotes the exchange process. Modification of the quenched-flow apparatus permitted measurement of the rate of nucleotide exchange during steady-state catalysis. The value of the first-order rate constant characterizing this process is similar to the catalytic rate constant determined under identical conditions. When MgATP is tightly bound to the enzyme, none of the kinetic properties of the enzyme described above were significantly changed. The results obtained suggest a mechanism in which two sites on the enzyme participate in catalysis. Several possible mechanisms consistent with the data are discussed.

Original languageEnglish (US)
Pages (from-to)2306-2312
Number of pages7
JournalBiochemistry
Volume26
Issue number8
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

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Chloroplast Proton-Translocating ATPases
Hydrolysis
Nucleotides
Adenosine Triphosphate
Binding Sites
Rate constants
Enzymes
Catalysis
Adenosine Diphosphate
Ion exchange
Kinetics
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Interactions between Nucleotide Binding Sites on Chloroplast Coupling Factor 1 during ATP Hydrolysis. / Leckband, Deborah E; Hammes, Gordon G.

In: Biochemistry, Vol. 26, No. 8, 01.01.1987, p. 2306-2312.

Research output: Contribution to journalArticle

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