Interactions between Clostridium beijerinckii and Geobacter metallireducens in co-culture fermentation with anthrahydroquinone-2, 6-disulfonate (AH2QDS) for enhanced biohydrogen production from xylose

Xinyu Zhang, Xiaofeng Ye, Kevin T. Finneran, Julie Zilles, Eberhard Morgenroth

Research output: Contribution to journalArticlepeer-review

Abstract

To enhance biohydrogen production, Clostridium beijerinckii was co-cultured with Geobacter metallireducens in the presence of the reduced extracellular electron shuttle anthrahydroquinone-2, 6-disulfonate (AH2QDS). In the co-culture system, increases of up to 52.3% for maximum cumulative hydrogen production, 38.4% for specific hydrogen production rate, 15.4% for substrate utilization rate, 39.0% for substrate utilization extent, and 34.8% for hydrogen molar yield in co-culture fermentation were observed compared to a pure culture of C. beijerinckii without AH2QDS. G. metallireducens grew in the co-culture system, resulting in a decrease in acetate concentration under co-culture conditions and a presumed regeneration of AH2QDS from AQDS. These co-culture results demonstrate metabolic crosstalk between the fermentative bacterium C. beijerinckii and the respiratory bacterium G. metallireducens and suggest a strategy for industrial biohydrogen production.

Original languageEnglish (US)
Pages (from-to)164-172
Number of pages9
JournalBiotechnology and bioengineering
Volume110
Issue number1
DOIs
StatePublished - Jan 2013

Keywords

  • Co-culture fermentation
  • Extracellular electron shuttles
  • Hydrogen production
  • Syntrophy
  • Xylose

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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