Inter-lab testing of Hyalella azteca water and sediment methods 2: Results from 10- to 42-d tests conducted with water-only and sediment substrates

T.J. Norberg-King, J.R. Hockett, D. R. Mount, D.J. Soucek, C. G. Ingersoll, C.D. Ivey, B.G. Brumbaugh, L.N. Taylor

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Over the past four years, USEPA Duluth, USGS Columbia, the Illinois Natural History Survey, and Environment Canada have been conduct-ing studies to refine the USEPA and ASTM International methods for conducting 10- to 42-d water-only or sediment toxicity exposures with the amphipod Hyalella azteca. Notable among these changes are the development of alternate feeding regimes to provide more robust growth and reproduction, as well as specifications on the composition of overlying water. To evaluate the effectiveness of these methods across laboratories, an inter-laboratory study involving 25 laboratories was organized with goals to: (1) determine whether the proposed new diets and water requirements will result in strong growth/reproduction of H. azteca in exposures across a range of laboratories, (2) determine whether use of the new diets/water will support increases in minimum control performance criteria for weight and reproduction, (3) determine if there are other diets or waters that are better than those being proposed. The number of treatments tested in each lab was variable, but all labs were asked to prioritize two treatments: (1) a diet of diatoms (Thalassiosira) and flaked fish food (Tetramin™) with rations of both increased weekly; and (2) a diet of the same rations of Tetramin™ combined with a fixed ration of yeast/cereal grass leaves/trout chow (YCT). Both diets were to be tested over a sand substrate and using a water containing ≥15 mg Cl/L and ≥0.02 mg Br/L. Beyond these two basic treatments, participating labs were also encouraged to test alternate substrates, diets, or waters of interest to the participating laboratory. All treatments involved testing 16 replicates beakers, with 4 replicates dedicated to survival and growth measurement at Day 10, 4 replicates dedicated to survival and growth measurement at Day 28, and the remaining 8 replicates for measurement of survival, growth, and reproduction through Day 42. This presentation will provide an overview of the data from the 25 participating laborato-ries and will evaluate these data in the context of draft modifications to the USEPA and ASTM H. azteca 10- to 42-d sediment or water toxicity testing methods
Original languageEnglish (US)
Title of host publicationSociety of Environmental Toxicology and Chemistry North America 35th Annual Meeting, 9-13 November 2014, Vancouver, British Columbia, Canada
Pages175
StatePublished - 2014

Keywords

  • INHS

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