Abstract
Neuropeptides support inter-cell communication and have a role in many diverse biological processes. For pig, a biomedical model, few prohormones from which neuropeptides result after convertase processing are listed in the UniProt database. Therefore, our goals are to obtain an in silico library of pig prohormone and convertase genes and to functionally annotate these genes based on a large number of complementary gene expression microarray experiments. Using a bioinformatics pipeline, 101 prohormone genes and 8 convertase genes known in human, rat, mouse, chicken, and cow were located in the pig genome. Frequently (P-value < 0.005) differentially expressed prohormone genes included adrenomedullin (ADML), augurin (AUGN), neuropeptide Y (NPY), proenkephalin-A (PENK), parathyroid hormone-related protein (PTHR), and vascular endothelial growth factor C (VEGFC) and convertases (PCSK1 and PCSK7). Embryo and placental tissues displayed the most differentially expressed genes. Our genomic characterization allows the use of the pig as an effective animal model to gain a deeper understanding of neuropeptides.
| Original language | English (US) |
|---|---|
| Title of host publication | 2011 IEEE International Conference on Bioinformatics and Biomedicine Workshops, BIBMW 2011 |
| Pages | 988-990 |
| Number of pages | 3 |
| DOIs | |
| State | Published - Dec 1 2011 |
| Event | 2011 IEEE International Conference onBioinformatics and Biomedicine Workshops, BIBMW 2011 - Atlanta, GA, United States Duration: Nov 12 2011 → Nov 15 2011 |
Publication series
| Name | 2011 IEEE International Conference on Bioinformatics and Biomedicine Workshops, BIBMW 2011 |
|---|
Other
| Other | 2011 IEEE International Conference onBioinformatics and Biomedicine Workshops, BIBMW 2011 |
|---|---|
| Country | United States |
| City | Atlanta, GA |
| Period | 11/12/11 → 11/15/11 |
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ASJC Scopus subject areas
- Biomedical Engineering
- Health Informatics
- Health Information Management
Cite this
Integration of sequence and functional information to identify and annotate neuropeptides in the pig genome. / Porter, Kenneth I.; Southey, Bruce R.; Akhtar, Malik N.; Sweedler, Jonathan V; Rodriguez-Zas, Sandra Luisa.
2011 IEEE International Conference on Bioinformatics and Biomedicine Workshops, BIBMW 2011. 2011. p. 988-990 6112532 (2011 IEEE International Conference on Bioinformatics and Biomedicine Workshops, BIBMW 2011).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Integration of sequence and functional information to identify and annotate neuropeptides in the pig genome
AU - Porter, Kenneth I.
AU - Southey, Bruce R.
AU - Akhtar, Malik N.
AU - Sweedler, Jonathan V
AU - Rodriguez-Zas, Sandra Luisa
PY - 2011/12/1
Y1 - 2011/12/1
N2 - Neuropeptides support inter-cell communication and have a role in many diverse biological processes. For pig, a biomedical model, few prohormones from which neuropeptides result after convertase processing are listed in the UniProt database. Therefore, our goals are to obtain an in silico library of pig prohormone and convertase genes and to functionally annotate these genes based on a large number of complementary gene expression microarray experiments. Using a bioinformatics pipeline, 101 prohormone genes and 8 convertase genes known in human, rat, mouse, chicken, and cow were located in the pig genome. Frequently (P-value < 0.005) differentially expressed prohormone genes included adrenomedullin (ADML), augurin (AUGN), neuropeptide Y (NPY), proenkephalin-A (PENK), parathyroid hormone-related protein (PTHR), and vascular endothelial growth factor C (VEGFC) and convertases (PCSK1 and PCSK7). Embryo and placental tissues displayed the most differentially expressed genes. Our genomic characterization allows the use of the pig as an effective animal model to gain a deeper understanding of neuropeptides.
AB - Neuropeptides support inter-cell communication and have a role in many diverse biological processes. For pig, a biomedical model, few prohormones from which neuropeptides result after convertase processing are listed in the UniProt database. Therefore, our goals are to obtain an in silico library of pig prohormone and convertase genes and to functionally annotate these genes based on a large number of complementary gene expression microarray experiments. Using a bioinformatics pipeline, 101 prohormone genes and 8 convertase genes known in human, rat, mouse, chicken, and cow were located in the pig genome. Frequently (P-value < 0.005) differentially expressed prohormone genes included adrenomedullin (ADML), augurin (AUGN), neuropeptide Y (NPY), proenkephalin-A (PENK), parathyroid hormone-related protein (PTHR), and vascular endothelial growth factor C (VEGFC) and convertases (PCSK1 and PCSK7). Embryo and placental tissues displayed the most differentially expressed genes. Our genomic characterization allows the use of the pig as an effective animal model to gain a deeper understanding of neuropeptides.
UR - http://www.scopus.com/inward/record.url?scp=84856008465&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84856008465&partnerID=8YFLogxK
U2 - 10.1109/BIBMW.2011.6112532
DO - 10.1109/BIBMW.2011.6112532
M3 - Conference contribution
AN - SCOPUS:84856008465
SN - 9781457716133
T3 - 2011 IEEE International Conference on Bioinformatics and Biomedicine Workshops, BIBMW 2011
SP - 988
EP - 990
BT - 2011 IEEE International Conference on Bioinformatics and Biomedicine Workshops, BIBMW 2011
ER -