Integration host factor facilitates repression of the put operon in Salmonella typhimurium

Kathryn O'Brien, Gregory Deno, Paula Ostrovsky de Spicer, Jeffrey F. Gardner, Stanley R. Maloy

Research output: Contribution to journalArticlepeer-review


Transcriptional regulation of the put operon is mediated by a unique mechanism involving autogenous regulation by the PutA protein, a membrane-associated dehydrogenase. The 420-bp put control region contains the putP and putA promoters, multiple operator sites, multiple catabolite repression protein binding sites, and several potential integration host factor (IHF)-binding sites (ihf). In this study, we show that IHF facilitates repression of the put operon in vivo, and IHF binds specifically to two ihf sites in the put control region in vitro. DNA gyrase mutants that alter the degree of chromosomal supercoiling do not affect put regulation, indicating that the effect of IHF on put expression is in this case independent of supercoiling.

Original languageEnglish (US)
Pages (from-to)13-19
Number of pages7
Issue number1
StatePublished - Sep 1 1992


  • IHF
  • him mutants
  • ihf sites
  • proline utilization
  • recombinant DNA

ASJC Scopus subject areas

  • Genetics


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