Insulin prohormone processing, distribution, and relation to metabolism in Aplysia californica

Philip D. Floyd; Lingjun Li; Stanislav S. Rubakhin; Jonathan V. Sweedler; Charles C. Horn; Irving Kupfermann; Vera Y. Alexeeva; Timothy A. Ellis; Nikolai C. Dembrow; Klaudiusz R. Weiss; Ferdinand S. Vilim

doi:10.1523/jneurosci.19-18-07732.1999

Insulin prohormone processing, distribution, and relation to metabolism in Aplysia californica

Philip D. Floyd, Lingjun Li, Stanislav S. Rubakhin, Jonathan V. Sweedler, Charles C. Horn, Irving Kupfermann, Vera Y. Alexeeva, Timothy A. Ellis, Nikolai C. Dembrow, Klaudiusz R. Weiss, Ferdinand S. Vilim

Research output: Contribution to journal › Article › peer-review

Abstract

The first Aplysia californica insulin gene is characterized and its proteolytic processing from prohormone to final peptides elucidated using a combination of biochemical and mass spectrometric methods. Aplysia insulin (AI) is one of the largest insulins found, with a molecular weight of 9146 Da, and an extended A chain compared with other invertebrate and vertebrate insulins. The AI prohormone produces a series of C peptides and also a unique N-terminally acetylated D peptide. AI-producing cells are restricted to the central region of the cerebral ganglia mostly within the F and C clusters, and AI is transported to neurohemal release sites located on the upper labial and anterior tentacular nerves. The expression of AI mRNA decreases when the animal is deprived of food, and injections of AI reduce hemolymph glucose levels, suggesting that the function of insulin-regulating metabolism has been conserved.

Original language	English (US)
Pages (from-to)	7732-7741
Number of pages	10
Journal	Journal of Neuroscience
Volume	19
Issue number	18
DOIs	https://doi.org/10.1523/jneurosci.19-18-07732.1999
State	Published - Sep 15 1999

Keywords

Aplysia californica
Cerebral ganglion
Glucose
Hunger/satiation
Insulin
Neuropeptide

ASJC Scopus subject areas

General Neuroscience

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10.1523/jneurosci.19-18-07732.1999

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title = "Insulin prohormone processing, distribution, and relation to metabolism in Aplysia californica",

abstract = "The first Aplysia californica insulin gene is characterized and its proteolytic processing from prohormone to final peptides elucidated using a combination of biochemical and mass spectrometric methods. Aplysia insulin (AI) is one of the largest insulins found, with a molecular weight of 9146 Da, and an extended A chain compared with other invertebrate and vertebrate insulins. The AI prohormone produces a series of C peptides and also a unique N-terminally acetylated D peptide. AI-producing cells are restricted to the central region of the cerebral ganglia mostly within the F and C clusters, and AI is transported to neurohemal release sites located on the upper labial and anterior tentacular nerves. The expression of AI mRNA decreases when the animal is deprived of food, and injections of AI reduce hemolymph glucose levels, suggesting that the function of insulin-regulating metabolism has been conserved.",

keywords = "Aplysia californica, Cerebral ganglion, Glucose, Hunger/satiation, Insulin, Neuropeptide",

author = "Floyd, {Philip D.} and Lingjun Li and Rubakhin, {Stanislav S.} and Sweedler, {Jonathan V.} and Horn, {Charles C.} and Irving Kupfermann and Alexeeva, {Vera Y.} and Ellis, {Timothy A.} and Dembrow, {Nikolai C.} and Weiss, {Klaudiusz R.} and Vilim, {Ferdinand S.}",

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AU - Li, Lingjun

AU - Rubakhin, Stanislav S.

AU - Sweedler, Jonathan V.

AU - Horn, Charles C.

AU - Kupfermann, Irving

AU - Alexeeva, Vera Y.

AU - Ellis, Timothy A.

AU - Dembrow, Nikolai C.

AU - Weiss, Klaudiusz R.

AU - Vilim, Ferdinand S.

PY - 1999/9/15

Y1 - 1999/9/15

N2 - The first Aplysia californica insulin gene is characterized and its proteolytic processing from prohormone to final peptides elucidated using a combination of biochemical and mass spectrometric methods. Aplysia insulin (AI) is one of the largest insulins found, with a molecular weight of 9146 Da, and an extended A chain compared with other invertebrate and vertebrate insulins. The AI prohormone produces a series of C peptides and also a unique N-terminally acetylated D peptide. AI-producing cells are restricted to the central region of the cerebral ganglia mostly within the F and C clusters, and AI is transported to neurohemal release sites located on the upper labial and anterior tentacular nerves. The expression of AI mRNA decreases when the animal is deprived of food, and injections of AI reduce hemolymph glucose levels, suggesting that the function of insulin-regulating metabolism has been conserved.

AB - The first Aplysia californica insulin gene is characterized and its proteolytic processing from prohormone to final peptides elucidated using a combination of biochemical and mass spectrometric methods. Aplysia insulin (AI) is one of the largest insulins found, with a molecular weight of 9146 Da, and an extended A chain compared with other invertebrate and vertebrate insulins. The AI prohormone produces a series of C peptides and also a unique N-terminally acetylated D peptide. AI-producing cells are restricted to the central region of the cerebral ganglia mostly within the F and C clusters, and AI is transported to neurohemal release sites located on the upper labial and anterior tentacular nerves. The expression of AI mRNA decreases when the animal is deprived of food, and injections of AI reduce hemolymph glucose levels, suggesting that the function of insulin-regulating metabolism has been conserved.

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KW - Cerebral ganglion

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KW - Hunger/satiation

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KW - Neuropeptide

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Insulin prohormone processing, distribution, and relation to metabolism in Aplysia californica

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