TY - JOUR
T1 - Insulin-like growth factor binding proteins in cerebrospinal fluid during human development and aging
AU - Arnold, Paul M.
AU - Ma, Jianxin Y.
AU - Citron, Bruce A.
AU - Festoff, Barry W.
N1 - Funding Information:
Support for these studies came from the American Parapolegia Society; University of Kansas Medical Center Alzheimer’s Disease Center Core grant (NIH P30 AG10182); the ALS/Spinal Cord Research Fund of the Kansas University Endowment Association; Cephalon, Inc.; and the Medical Research Service of the Department of Veterans Affairs.
PY - 1999/11/2
Y1 - 1999/11/2
N2 - We analyzed samples of insulin-like growth factor binding proteins (IGFBPs) in human cerebrospinal fluid (CSF) in neurologically normal patients from one day after birth to age 76 years. CSF samples were separated on SDS-PAGE and then transferred to nitrocellulose membranes where IGFBPs were detected by Western ligand blot using [125I]-IGF-II, confirming other reports where we found the presence of IGFBP-2, 3, 4, 5. The 34 kDa IGFBP-2 was present in all samples, and progressively decreased with age. A broad 28- to 30-kDa IGFBP band, having the appearance of IGFBP-5, was triphasic: faint during infancy, barely detectable at 6 months, but intense in adult and aged individuals. The 24-kDa IGFBP-4 band was only seen in neonatal CSF samples, while the IGFBP-3 doublet gradually increased during aging. Thus, these present results show that IGFBP-2, 3, 4 and 5 in CSF are developmentally regulated, suggesting roles for these molecules in the development of the nervous system.
AB - We analyzed samples of insulin-like growth factor binding proteins (IGFBPs) in human cerebrospinal fluid (CSF) in neurologically normal patients from one day after birth to age 76 years. CSF samples were separated on SDS-PAGE and then transferred to nitrocellulose membranes where IGFBPs were detected by Western ligand blot using [125I]-IGF-II, confirming other reports where we found the presence of IGFBP-2, 3, 4, 5. The 34 kDa IGFBP-2 was present in all samples, and progressively decreased with age. A broad 28- to 30-kDa IGFBP band, having the appearance of IGFBP-5, was triphasic: faint during infancy, barely detectable at 6 months, but intense in adult and aged individuals. The 24-kDa IGFBP-4 band was only seen in neonatal CSF samples, while the IGFBP-3 doublet gradually increased during aging. Thus, these present results show that IGFBP-2, 3, 4 and 5 in CSF are developmentally regulated, suggesting roles for these molecules in the development of the nervous system.
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U2 - 10.1006/bbrc.1999.1555
DO - 10.1006/bbrc.1999.1555
M3 - Article
C2 - 10543987
AN - SCOPUS:0033517822
SN - 0006-291X
VL - 264
SP - 652
EP - 656
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -