Instantaneous spatial light interference microscopy

Huafeng Ding, Gabriel Popescu

Research output: Contribution to journalArticle


We present Instantaneous Spatial Light Interference Microscopy (iSLIM) as a new quantitative phase method that combines the benefits of white light illumination in Zernike's phase contrast microscopy and phase stability associated diffraction phase microscopy. iSLIM is implemented as an add-on module to a commercial phase contrast microscope, and enables new features to quantitative phase imaging: diminished speckle effects due to white light illumination, multimodal investigation potential due to overlaying with other modalities of the microscope (e.g. fluorescence, DIC, phase contrast), and spectroscopic potential due to the broad band light. We show proof of principle results by multicolor phase imaging of microsphere and red blood cells, and dynamic imaging of nanoscale cell membrane fluctuations.

Original languageEnglish (US)
Pages (from-to)1569-1575
Number of pages7
JournalOptics Express
Issue number2
StatePublished - Jan 18 2010

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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