@article{37979b43b68a48cdad4f86e1b1106ba1,
title = "Insights into AMS/PCAT transporters from biochemical and structural characterization of a double glycine motif protease",
abstract = "The secretion of peptides and proteins is essential for survival and ecological adaptation of bacteria. Dual-functional ATP-binding cassette transporters export antimicrobial or quorum signaling peptides in Gram-positive bacteria. Their substrates contain a leader sequence that is excised by an N-terminal peptidase C39 domain at a double Gly motif. We characterized the protease domain (LahT150) of a transporter from a lanthipeptide biosynthetic operon in Lachnospiraceae and demonstrate that this protease can remove the leader peptide from a diverse set of peptides. The 2.0 {\AA} resolution crystal structure of the protease domain in complex with a covalently bound leader peptide demonstrates the basis for substrate recognition across the entire class of such transporters. The structural data also provide a model for understanding the role of leader peptide recognition in the translocation cycle, and the function of degenerate, non-functional C39-like domains (CLD) in substrate recruitment in toxin exporters in Gram-negative bacteria.",
author = "Bobeica, {Silvia C.} and Dong, {Shi Hui} and Liujie Huo and Nuria Mazo and McLaughlin, {Martin I.} and Gonzalo Jim{\'e}nez-Os{\'e}s and Nair, {Satish K.} and {van der Donk}, {Wilfred A.}",
note = "Funding Information: This work was supported by grants from the National Institutes of Health (GM R37 058822 to WAV and GM079038 to SKN), and MINECO/FEDER (CTQ2015-70524-R and RYC-2013–14706 grants to GJO). NM acknowledges Universidad de La Rioja for a predoctoral fellowship. We thank Dr. William Kelly (AgResearch, New Zealand) for providing Lachnospiraceae bacterium C6A11, and Keith Brister and colleagues for facilitating data collection at LS-CAT (Argonne National Labs, IL). Funding Information: This work was supported by grants from the National Institutes of Health (GM R37 058822 to WAV and GM079038 to SKN), and MINECO/FEDER (CTQ2015-70524-R and RYC-2013?14706 grants to GJO). NM acknowledges Universidad de La Rioja for a predoctoral fellowship. We thank Dr. William Kelly (AgResearch, New Zealand) for providing Lachnospiraceae bacterium C6A11, and Keith Brister and colleagues for facilitating data collection at LS-CAT (Argonne National Labs, IL). Publisher Copyright: {\textcopyright} Bobeica et al.",
year = "2019",
doi = "10.7554/eLife.42305",
language = "English (US)",
volume = "8",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}