Abstract
Single-cell explorations offer a unique window to inspect molecules and events relevant to mechanisms and heterogeneity constituting the central dogma of biology. A large number of nucleic acids, proteins, metabolites, and small molecules are involved in determining and fine-tuning the state and function of a single cell at a given time point. Advanced optical platforms and nanotools provide tremendous opportunities to probe intracellular components with single-molecule accuracy, as well as promising tools to adjust single-cell activity. To obtain quantitative information (e.g., molecular quantity, kinetics, and stoichiometry) within an intact cell, achieving the observation with comparable spatiotemporal resolution is a challenge. For single-cell studies, both the method of detection and the biocompatibility are critical factors as they determine the feasibility, especially when considering live-cell analysis. Although a considerable proportion of single-cell methodologies depend on specialized expertise and expensive instruments, it is our expectation that the information content and implication will outweigh the costs given the impact on life science enabled by single-cell analysis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 387-407 |
| Number of pages | 21 |
| Journal | Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology |
| Volume | 7 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 1 2015 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Bioengineering
- Medicine (miscellaneous)
- Biomedical Engineering
Cite this
Inside single cells : Quantitative analysis with advanced optics and nanomaterials. / Cui, Yi; Irudayaraj, Joseph.
In: Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, Vol. 7, No. 3, 01.05.2015, p. 387-407.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Inside single cells
T2 - Quantitative analysis with advanced optics and nanomaterials
AU - Cui, Yi
AU - Irudayaraj, Joseph
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Single-cell explorations offer a unique window to inspect molecules and events relevant to mechanisms and heterogeneity constituting the central dogma of biology. A large number of nucleic acids, proteins, metabolites, and small molecules are involved in determining and fine-tuning the state and function of a single cell at a given time point. Advanced optical platforms and nanotools provide tremendous opportunities to probe intracellular components with single-molecule accuracy, as well as promising tools to adjust single-cell activity. To obtain quantitative information (e.g., molecular quantity, kinetics, and stoichiometry) within an intact cell, achieving the observation with comparable spatiotemporal resolution is a challenge. For single-cell studies, both the method of detection and the biocompatibility are critical factors as they determine the feasibility, especially when considering live-cell analysis. Although a considerable proportion of single-cell methodologies depend on specialized expertise and expensive instruments, it is our expectation that the information content and implication will outweigh the costs given the impact on life science enabled by single-cell analysis.
AB - Single-cell explorations offer a unique window to inspect molecules and events relevant to mechanisms and heterogeneity constituting the central dogma of biology. A large number of nucleic acids, proteins, metabolites, and small molecules are involved in determining and fine-tuning the state and function of a single cell at a given time point. Advanced optical platforms and nanotools provide tremendous opportunities to probe intracellular components with single-molecule accuracy, as well as promising tools to adjust single-cell activity. To obtain quantitative information (e.g., molecular quantity, kinetics, and stoichiometry) within an intact cell, achieving the observation with comparable spatiotemporal resolution is a challenge. For single-cell studies, both the method of detection and the biocompatibility are critical factors as they determine the feasibility, especially when considering live-cell analysis. Although a considerable proportion of single-cell methodologies depend on specialized expertise and expensive instruments, it is our expectation that the information content and implication will outweigh the costs given the impact on life science enabled by single-cell analysis.
UR - http://www.scopus.com/inward/record.url?scp=84927155061&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84927155061&partnerID=8YFLogxK
U2 - 10.1002/wnan.1321
DO - 10.1002/wnan.1321
M3 - Review article
C2 - 25430077
AN - SCOPUS:84927155061
VL - 7
SP - 387
EP - 407
JO - Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology
JF - Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology
SN - 1939-0041
IS - 3
ER -