Insertional restoration of β-galactosidase α-complementation (white-to-blue colony screening) facilitates assembly of synthetic genes

Keyphrases

• DNA Segment 100%
• Synthetic Sequences 100%
• β-Galactosidase (β-Gal) 100%
• Synthetic Gene 100%
• Insertional 100%
• Colony Screening 100%
• Plasmid 66%
• Synthetic DNA 66%
• Frameshift 66%
• DNA Sequencing 33%
• Escherichia Coli 33%
• Oligodeoxynucleotide 33%
• Upstream Open Reading Frame (uORF) 33%
• LacZ 33%
• Ligation 33%
• Screening Method 33%
• Digestion 33%
• Phage 33%
• Restriction Sites 33%
• Plasmid Vector 33%
• Reading Frame 33%
• Recombinant Plasmid 33%
• DNA Insertion 33%
• Gene Segment 33%
• White Noise Test 33%
• Biological Test 33%
• Cohesive Ends 33%
• LacZ Gene 33%
• Plasmid Transformation 33%
• Insertional Inactivation 33%
• Proper Length 33%

Biochemistry, Genetics and Molecular Biology

• Plasmid 100%
• Galactosidase 100%
• DNA Sequence 50%
• Escherichia coli 50%
• Open Reading Frame 50%
• Screening 50%
• Bacteriophage 50%
• Restriction Site 50%
• Reading Frame 50%
• Recombinant Plasmid 50%
• Gene Insertion 50%
• Plasmid Vector 50%
• Gene Segment 50%