TY - JOUR
T1 - Inorganic Mercury Detection and Controlled Release of Chelating Agents from Ion-Responsive Liposomes
AU - Yigit, Mehmet Veysel
AU - Mishra, Abhijit
AU - Tong, Rong
AU - Cheng, Jianjun
AU - Wong, Gerard C.L.
AU - Lu, Yi
N1 - This work was supported by the US National Science Foundation (CTS-0120978 and DMR-0117792) and the Department of Energy (DE-FG02-08ER64568).
PY - 2009/9/25
Y1 - 2009/9/25
N2 - A liposome system that can detect and detoxify mercury in aqueous solution is demonstrated. The system consists of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine and 20% PEG-PE (PEG MW 2000 Da) that forms liposome, which encapsulates self-quenching fluorescein for detection, and chelating agent meso-2,3-dimercaptosuccinic acid (meso-DMSA) for chelating detoxification through Hg2+-responsive release of fluorescein and meso-DMSA. This system can detect mercury levels as low as 10 nM with high selectivity. In particular, the release profile of meso-DMSA by the local concentration of Hg can be modulated, so that more chelators are released in regions of high concentration and less chelators are released in regions of low concentration. The design has been demonstrated both in vitro and in HeLa cells. This "budgeted" release profile is particularly useful in situations in which the local levels of Hg contamination vary, or if such contamination is time dependent.
AB - A liposome system that can detect and detoxify mercury in aqueous solution is demonstrated. The system consists of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine and 20% PEG-PE (PEG MW 2000 Da) that forms liposome, which encapsulates self-quenching fluorescein for detection, and chelating agent meso-2,3-dimercaptosuccinic acid (meso-DMSA) for chelating detoxification through Hg2+-responsive release of fluorescein and meso-DMSA. This system can detect mercury levels as low as 10 nM with high selectivity. In particular, the release profile of meso-DMSA by the local concentration of Hg can be modulated, so that more chelators are released in regions of high concentration and less chelators are released in regions of low concentration. The design has been demonstrated both in vitro and in HeLa cells. This "budgeted" release profile is particularly useful in situations in which the local levels of Hg contamination vary, or if such contamination is time dependent.
KW - CHEMBIO
UR - https://www.scopus.com/pages/publications/70349190310
UR - https://www.scopus.com/pages/publications/70349190310#tab=citedBy
U2 - 10.1016/j.chembiol.2009.08.011
DO - 10.1016/j.chembiol.2009.08.011
M3 - Article
C2 - 19778721
AN - SCOPUS:70349190310
SN - 1074-5521
VL - 16
SP - 937
EP - 942
JO - Chemistry and Biology
JF - Chemistry and Biology
IS - 9
ER -