Inoculation with an enhanced N2-fixing Bradyrhizobium japonicum strain (USDA110) does not alter soybean (Glycine max Merr.) response to elevated [CO2]

Álvaro Sanz-sáez, Katy Denise Heath, Patricia V. Burke, Elizabeth Ainsworth

Research output: Contribution to journalArticle

Abstract

This study tested the hypothesis that inoculation of soybean (Glycine maxMerr.) with a Bradyrhizobium japonicum strain (USDA110) with greater N2 fixation rates would enhance soybean response to elevated [CO2]. In field experiments at the Soybean Free Air CO2 Enrichment facility, inoculation of soybean with USDA110 increased nodule occupancy from 5% in native soil to 54% in elevated [CO2] and 34% at ambient [CO2]. Despite this success, inoculation with USDA110 did not result in greater photosynthesis, growth or seed yield at ambient or elevated [CO2] in the field, presumably due to competition from native rhizobia. In a growth chamber experiment designed to study the effects of inoculation in the absence of competition, inoculation with USDA110 in sterilized soil resulted in nodule occupation of >90%, significantly greater 15N2 fixation, photosynthetic capacity, leaf N and total plant biomass compared with plants grown with native soil bacteria. However, there was no interaction of rhizobium fertilization with elevated [CO2]; inoculation with USDA110 was equally beneficial at ambient and elevated [CO2]. These results suggest that selected rhizobia could potentially stimulate soybean yield in soils with little or no history of prior soybean production, but that better quality rhizobia do not enhance soybean responses to elevated [CO2].

Original languageEnglish (US)
Pages (from-to)2589-2602
Number of pages14
JournalPlant Cell and Environment
Volume38
Issue number12
DOIs
StatePublished - Dec 1 2015

Fingerprint

Bradyrhizobium
Bradyrhizobium japonicum
Soybeans
Glycine max
carbon dioxide
Rhizobium
soybeans
Soil
Nitrogen Fixation
Glycine (Fabaceae)
soil
Photosynthesis
Growth
Occupations
Fertilization
soil bacteria
Biomass
Glycine
growth chambers
Seeds

Keywords

  • Elevated carbon dioxide
  • Free air CO enrichment
  • N fixation
  • Nodulation
  • Rhizobia

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

@article{b8bdbb2f1dbe4a658a5e765f0f9af27e,
title = "Inoculation with an enhanced N2-fixing Bradyrhizobium japonicum strain (USDA110) does not alter soybean (Glycine max Merr.) response to elevated [CO2]",
abstract = "This study tested the hypothesis that inoculation of soybean (Glycine maxMerr.) with a Bradyrhizobium japonicum strain (USDA110) with greater N2 fixation rates would enhance soybean response to elevated [CO2]. In field experiments at the Soybean Free Air CO2 Enrichment facility, inoculation of soybean with USDA110 increased nodule occupancy from 5{\%} in native soil to 54{\%} in elevated [CO2] and 34{\%} at ambient [CO2]. Despite this success, inoculation with USDA110 did not result in greater photosynthesis, growth or seed yield at ambient or elevated [CO2] in the field, presumably due to competition from native rhizobia. In a growth chamber experiment designed to study the effects of inoculation in the absence of competition, inoculation with USDA110 in sterilized soil resulted in nodule occupation of >90{\%}, significantly greater 15N2 fixation, photosynthetic capacity, leaf N and total plant biomass compared with plants grown with native soil bacteria. However, there was no interaction of rhizobium fertilization with elevated [CO2]; inoculation with USDA110 was equally beneficial at ambient and elevated [CO2]. These results suggest that selected rhizobia could potentially stimulate soybean yield in soils with little or no history of prior soybean production, but that better quality rhizobia do not enhance soybean responses to elevated [CO2].",
keywords = "Elevated carbon dioxide, Free air CO enrichment, N fixation, Nodulation, Rhizobia",
author = "{\'A}lvaro Sanz-s{\'a}ez and Heath, {Katy Denise} and Burke, {Patricia V.} and Elizabeth Ainsworth",
year = "2015",
month = "12",
day = "1",
doi = "10.1111/pce.12577",
language = "English (US)",
volume = "38",
pages = "2589--2602",
journal = "Plant, Cell and Environment",
issn = "0140-7791",
publisher = "Wiley-Blackwell",
number = "12",

}

TY - JOUR

T1 - Inoculation with an enhanced N2-fixing Bradyrhizobium japonicum strain (USDA110) does not alter soybean (Glycine max Merr.) response to elevated [CO2]

AU - Sanz-sáez, Álvaro

AU - Heath, Katy Denise

AU - Burke, Patricia V.

AU - Ainsworth, Elizabeth

PY - 2015/12/1

Y1 - 2015/12/1

N2 - This study tested the hypothesis that inoculation of soybean (Glycine maxMerr.) with a Bradyrhizobium japonicum strain (USDA110) with greater N2 fixation rates would enhance soybean response to elevated [CO2]. In field experiments at the Soybean Free Air CO2 Enrichment facility, inoculation of soybean with USDA110 increased nodule occupancy from 5% in native soil to 54% in elevated [CO2] and 34% at ambient [CO2]. Despite this success, inoculation with USDA110 did not result in greater photosynthesis, growth or seed yield at ambient or elevated [CO2] in the field, presumably due to competition from native rhizobia. In a growth chamber experiment designed to study the effects of inoculation in the absence of competition, inoculation with USDA110 in sterilized soil resulted in nodule occupation of >90%, significantly greater 15N2 fixation, photosynthetic capacity, leaf N and total plant biomass compared with plants grown with native soil bacteria. However, there was no interaction of rhizobium fertilization with elevated [CO2]; inoculation with USDA110 was equally beneficial at ambient and elevated [CO2]. These results suggest that selected rhizobia could potentially stimulate soybean yield in soils with little or no history of prior soybean production, but that better quality rhizobia do not enhance soybean responses to elevated [CO2].

AB - This study tested the hypothesis that inoculation of soybean (Glycine maxMerr.) with a Bradyrhizobium japonicum strain (USDA110) with greater N2 fixation rates would enhance soybean response to elevated [CO2]. In field experiments at the Soybean Free Air CO2 Enrichment facility, inoculation of soybean with USDA110 increased nodule occupancy from 5% in native soil to 54% in elevated [CO2] and 34% at ambient [CO2]. Despite this success, inoculation with USDA110 did not result in greater photosynthesis, growth or seed yield at ambient or elevated [CO2] in the field, presumably due to competition from native rhizobia. In a growth chamber experiment designed to study the effects of inoculation in the absence of competition, inoculation with USDA110 in sterilized soil resulted in nodule occupation of >90%, significantly greater 15N2 fixation, photosynthetic capacity, leaf N and total plant biomass compared with plants grown with native soil bacteria. However, there was no interaction of rhizobium fertilization with elevated [CO2]; inoculation with USDA110 was equally beneficial at ambient and elevated [CO2]. These results suggest that selected rhizobia could potentially stimulate soybean yield in soils with little or no history of prior soybean production, but that better quality rhizobia do not enhance soybean responses to elevated [CO2].

KW - Elevated carbon dioxide

KW - Free air CO enrichment

KW - N fixation

KW - Nodulation

KW - Rhizobia

UR - http://www.scopus.com/inward/record.url?scp=84951754116&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84951754116&partnerID=8YFLogxK

U2 - 10.1111/pce.12577

DO - 10.1111/pce.12577

M3 - Article

C2 - 26012898

AN - SCOPUS:84951754116

VL - 38

SP - 2589

EP - 2602

JO - Plant, Cell and Environment

JF - Plant, Cell and Environment

SN - 0140-7791

IS - 12

ER -