Inhibitory effects of wild blueberry anthocyanins and other flavonoids on biomarkers of acute and chronic inflammation in vitro

Debora Esposito; Amelia Chen; Mary H. Grace; Slavko Komarnytsky; Mary Ann Lila

doi:10.1021/jf4051599

Inhibitory effects of wild blueberry anthocyanins and other flavonoids on biomarkers of acute and chronic inflammation in vitro

Debora Esposito, Amelia Chen, Mary H. Grace, Slavko Komarnytsky, Mary Ann Lila

Research output: Contribution to journal › Article › peer-review

Abstract

Wild lowbush blueberries (Vaccinium angustifolium Ait) are a rich source of anthocyanins and other flavonoids with anti-inflammatory activities; however, their individual effects on cellular signaling remain to be elucidated. This study determined the capacity of blueberry bioactives to protect murine RAW 264.7 macrophages from lipopolysaccharide-induced inflammation. Fractionation of the crude extract (CE) into polyphenol-rich (PPR), anthocyanin-rich (ANC), and proanthocyanidin-rich (PAC) fractions and an ethyl acetate fraction (EA) revealed that PPR, ANC, and PAC components most effectively suppressed mRNA biomarkers of acute inflammation (Cox-2, iNOS, and IL-1β). Among major polyphenols found in the wild blueberries, malvidin-3-glucoside was significantly more effective than epicatechin or chlorogenic acid in reducing the expression of pro-inflammatory genes in vitro.

Original language	English (US)
Pages (from-to)	7022-7028
Number of pages	7
Journal	Journal of Agricultural and Food Chemistry
Volume	62
Issue number	29
DOIs	https://doi.org/10.1021/jf4051599
State	Published - Jul 23 2014
Externally published	Yes

Keywords

chlorogenic acid
lowbush blueberry
polyphenols
proanthocyanidins
Vaccinium angustifolium

ASJC Scopus subject areas

Chemistry(all)
Agricultural and Biological Sciences(all)

Online availability

10.1021/jf4051599

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title = "Inhibitory effects of wild blueberry anthocyanins and other flavonoids on biomarkers of acute and chronic inflammation in vitro",

abstract = "Wild lowbush blueberries (Vaccinium angustifolium Ait) are a rich source of anthocyanins and other flavonoids with anti-inflammatory activities; however, their individual effects on cellular signaling remain to be elucidated. This study determined the capacity of blueberry bioactives to protect murine RAW 264.7 macrophages from lipopolysaccharide-induced inflammation. Fractionation of the crude extract (CE) into polyphenol-rich (PPR), anthocyanin-rich (ANC), and proanthocyanidin-rich (PAC) fractions and an ethyl acetate fraction (EA) revealed that PPR, ANC, and PAC components most effectively suppressed mRNA biomarkers of acute inflammation (Cox-2, iNOS, and IL-1β). Among major polyphenols found in the wild blueberries, malvidin-3-glucoside was significantly more effective than epicatechin or chlorogenic acid in reducing the expression of pro-inflammatory genes in vitro.",

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author = "Debora Esposito and Amelia Chen and Grace, {Mary H.} and Slavko Komarnytsky and Lila, {Mary Ann}",

year = "2014",

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AU - Esposito, Debora

AU - Chen, Amelia

AU - Grace, Mary H.

AU - Komarnytsky, Slavko

AU - Lila, Mary Ann

PY - 2014/7/23

Y1 - 2014/7/23

N2 - Wild lowbush blueberries (Vaccinium angustifolium Ait) are a rich source of anthocyanins and other flavonoids with anti-inflammatory activities; however, their individual effects on cellular signaling remain to be elucidated. This study determined the capacity of blueberry bioactives to protect murine RAW 264.7 macrophages from lipopolysaccharide-induced inflammation. Fractionation of the crude extract (CE) into polyphenol-rich (PPR), anthocyanin-rich (ANC), and proanthocyanidin-rich (PAC) fractions and an ethyl acetate fraction (EA) revealed that PPR, ANC, and PAC components most effectively suppressed mRNA biomarkers of acute inflammation (Cox-2, iNOS, and IL-1β). Among major polyphenols found in the wild blueberries, malvidin-3-glucoside was significantly more effective than epicatechin or chlorogenic acid in reducing the expression of pro-inflammatory genes in vitro.

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KW - chlorogenic acid

KW - lowbush blueberry

KW - polyphenols

KW - proanthocyanidins

KW - Vaccinium angustifolium

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Inhibitory effects of wild blueberry anthocyanins and other flavonoids on biomarkers of acute and chronic inflammation in vitro

Abstract

Keywords

ASJC Scopus subject areas

Online availability

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