Inhibitory effect of a glycoprotein isolated from golden oyster mushroom (Pleurotus citrinopileatus) on the lipopolysaccharide-induced inflammatory reaction in RAW 264.7 macrophage

Jian Nan Chen, Elvira Gonzalez De Mejia, James Swi Bea Wu

Research output: Contribution to journalArticlepeer-review

Abstract

Mushrooms have become an important source of natural antitumor, antiviral, antibacterial, immunomodulatory, and anti-inflammatioy agents. Golden oyster mushroom, Pleurotus citrinopileatus, is a common mushroom in oriental countries for human consumption. The present study investigated the anti-inflammatory reaction of the bioactive nonlectin glycoprotein (PCP-3A) isolated from the fresh fruiting body of this mushroom. Western blot analysis on LPS-induced iNOS, COX-2, and NF-κB expressions in RAW 264.7 cells as affected by PCP3-A was performed to elucidate the mechanism of NO and PGE2 reduction. The results showed that PCP-3A failed to affect RAW 264.7 viability at a concentration up to 6.25 μg/mL, but inhibited LPS (1 μg/mL)-induced expression, and that PCP-3A inhibited the production of NO and PGE2 in LPS-activated macrophages via the down-regulation of certain pro-inflammatory mediators, including iNOS and NF-κB.

Original languageEnglish (US)
Pages (from-to)7092-7097
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number13
DOIs
StatePublished - Jul 13 2011

Keywords

  • PCP-3A
  • RAW 264.7 cell
  • glycoprotein
  • mushroom

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Fingerprint Dive into the research topics of 'Inhibitory effect of a glycoprotein isolated from golden oyster mushroom (Pleurotus citrinopileatus) on the lipopolysaccharide-induced inflammatory reaction in RAW 264.7 macrophage'. Together they form a unique fingerprint.

Cite this