Inhibition of Rat Mammary Tumorigenesis by Concord Grape Juice Constituents

Keith W. Singletary, Matthew J. Stansbury, Monica Giusti, Richard B. Van Breemen, Matthew Wallig, Agnes Rimando

Research output: Contribution to journalArticlepeer-review


The effects of Concord grape juice constituents on the promotion of chemically induced rat mammary tumor development and on the proliferation of a rat mammary adenocarcinoma cell line were studied. Isocaloric grape juice formulations provided in the drinking fluid of rats at concentrations of 489 and 651 mg of phenolics/dL of fluid significantly inhibited mammary adenocarcinoma multiplicity compared to controls. Final tumor mass also was significantly decreased for animals provided these two grape juice concentrations compared to controls. In addition, DNA synthesis of the rat mammary adenocarcinoma RBA cell line was significantly inhibited in a dose-dependent manner for cells treated with a grape extract, with an IC 50 dose of ∼14 μg of phenolics/mL. This inhibition of DNA synthesis was not accompanied by changes in 8-oxodeoxyguanosine formation or by substantial cell cycle arrest. These studies thus indicate that Concord grape juice constituents can inhibit the promotion stage of 7,12-dimethylbenz[a]anthracene (DMBA)-induced rat mammary tumorigenesis, in part by suppressing cell proliferation.

Original languageEnglish (US)
Pages (from-to)7280-7286
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Issue number25
StatePublished - Dec 3 2003


  • 7,12-dimethylbenz[a]anthracene
  • Breast cancer
  • Grape

ASJC Scopus subject areas

  • General Chemistry
  • General Agricultural and Biological Sciences


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