Inhibition of photosynthesis and respiration by substituted 2,6-dinitroaniline herbicides. III. Effects on electron transport and membrane properties of isolated mung bean mitochrondria

D. E. Moreland, Steven C Huber

Research output: Contribution to journalArticle

Abstract

Twelve substituted 2,6-dinitroaniline herbicides were shown to interfere with electron transport and phosphorylation in isolated mung bean (Phaseolus aureus Roxb.) mitochondria. Postulated sites of action involved complexes I and II, at or near the flavoprotein associated with the oxidation of exogenous NADH, the alternate (cyanide-insensitive) oxidase, and some step in the phosphorylation pathway. Differences among the various 2,6-dinitroanilines tested were generally quantitative rather than qualitative. All of the 2,6-dinitroanilines inhibited the rate of valinomycin-induced mitochondrial swelling in isotonic KCl within the same concentration range that caused inhibition of state 3 respiration. It is postulated that reduced membrane fluidity, or inhibition of chloride transport, may explain the effects on valinomycin-induced swelling. We propose that the 2,6-dinitroanilines partition into the inner mitochondrial membrane, thereby decrease membrane fluidity, and alter membrane permeability. The resulting perturbations to the structural organization of the electron transport components may be responsible for the apparent multiple-site inhibition of respiration.

Original languageEnglish (US)
Pages (from-to)247-257
Number of pages11
JournalPesticide Biochemistry and Physiology
Volume11
Issue number1-3
DOIs
StatePublished - Jul 1979

Fingerprint

dinitroaniline herbicides
valinomycin
Valinomycin
membrane fluidity
Membrane Fluidity
Photosynthesis
Herbicides
Electron Transport
mung beans
cell respiration
electron transfer
phosphorylation
Respiration
Phosphorylation
photosynthesis
Mitochondrial Swelling
NADH dehydrogenase (ubiquinone)
flavoproteins
Vigna radiata var. radiata
Flavoproteins

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis

Cite this

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title = "Inhibition of photosynthesis and respiration by substituted 2,6-dinitroaniline herbicides. III. Effects on electron transport and membrane properties of isolated mung bean mitochrondria",
abstract = "Twelve substituted 2,6-dinitroaniline herbicides were shown to interfere with electron transport and phosphorylation in isolated mung bean (Phaseolus aureus Roxb.) mitochondria. Postulated sites of action involved complexes I and II, at or near the flavoprotein associated with the oxidation of exogenous NADH, the alternate (cyanide-insensitive) oxidase, and some step in the phosphorylation pathway. Differences among the various 2,6-dinitroanilines tested were generally quantitative rather than qualitative. All of the 2,6-dinitroanilines inhibited the rate of valinomycin-induced mitochondrial swelling in isotonic KCl within the same concentration range that caused inhibition of state 3 respiration. It is postulated that reduced membrane fluidity, or inhibition of chloride transport, may explain the effects on valinomycin-induced swelling. We propose that the 2,6-dinitroanilines partition into the inner mitochondrial membrane, thereby decrease membrane fluidity, and alter membrane permeability. The resulting perturbations to the structural organization of the electron transport components may be responsible for the apparent multiple-site inhibition of respiration.",
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N2 - Twelve substituted 2,6-dinitroaniline herbicides were shown to interfere with electron transport and phosphorylation in isolated mung bean (Phaseolus aureus Roxb.) mitochondria. Postulated sites of action involved complexes I and II, at or near the flavoprotein associated with the oxidation of exogenous NADH, the alternate (cyanide-insensitive) oxidase, and some step in the phosphorylation pathway. Differences among the various 2,6-dinitroanilines tested were generally quantitative rather than qualitative. All of the 2,6-dinitroanilines inhibited the rate of valinomycin-induced mitochondrial swelling in isotonic KCl within the same concentration range that caused inhibition of state 3 respiration. It is postulated that reduced membrane fluidity, or inhibition of chloride transport, may explain the effects on valinomycin-induced swelling. We propose that the 2,6-dinitroanilines partition into the inner mitochondrial membrane, thereby decrease membrane fluidity, and alter membrane permeability. The resulting perturbations to the structural organization of the electron transport components may be responsible for the apparent multiple-site inhibition of respiration.

AB - Twelve substituted 2,6-dinitroaniline herbicides were shown to interfere with electron transport and phosphorylation in isolated mung bean (Phaseolus aureus Roxb.) mitochondria. Postulated sites of action involved complexes I and II, at or near the flavoprotein associated with the oxidation of exogenous NADH, the alternate (cyanide-insensitive) oxidase, and some step in the phosphorylation pathway. Differences among the various 2,6-dinitroanilines tested were generally quantitative rather than qualitative. All of the 2,6-dinitroanilines inhibited the rate of valinomycin-induced mitochondrial swelling in isotonic KCl within the same concentration range that caused inhibition of state 3 respiration. It is postulated that reduced membrane fluidity, or inhibition of chloride transport, may explain the effects on valinomycin-induced swelling. We propose that the 2,6-dinitroanilines partition into the inner mitochondrial membrane, thereby decrease membrane fluidity, and alter membrane permeability. The resulting perturbations to the structural organization of the electron transport components may be responsible for the apparent multiple-site inhibition of respiration.

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