Inhibition of growth of mouse gastric cancer cells by Runx3, a novel tumor suppressor

Wei Hui Guo, Li Qin Weng, Kosei Ito, Lin Feng Chen, Hayao Nakanishi, Masae Tatematsu, Yoshiaki Ito

Research output: Contribution to journalArticlepeer-review

Abstract

We reported recently that the silencing of RUNX3 is causally related to gastric cancer in humans. Here we report that in three of four cell lines derived from N-methyl-N-nitrosourea-induced mouse glandular stomach carcinomas, Runx3 is silenced due to hypermethylation of CpG islands in the promoter region, as we also observed for human gastric cancer cells. Although two of the sites we tested in the promoter of the fourth line were not methylated, in all four cases the silencing of Runx3 could be reversed by treatment of the cells with 5′-azacytidine and trichostatin A. Interestingly, the exogenous expression of RUNX3 in cell lines that do not express the endogenous gene caused an inhibition of growth in soft agar, suggesting that anchorage-independent growth could be used as an assay of RUNX3 activity in vitro. These observations suggest that the mouse system described here may be useful as a model for the study of human gastric carcinogenesis.

Original languageEnglish (US)
Pages (from-to)8351-8355
Number of pages5
JournalOncogene
Volume21
Issue number54
DOIs
StatePublished - Nov 28 2002
Externally publishedYes

Keywords

  • Gastric cancer
  • Methylation
  • Runx3
  • Tumor suppressor

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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