Inhibition of amino acid transport in Bacillus subtilis by uncouplers of oxidative phosphorylation

Thomas B. Brummett, George W. Ordal

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of three uncouplers of oxidative phosphorylation, trifluoromethoxycarbon-ylcyanidephenylhydrazone (FCCP), 3,3′,4′,5-tetrachlorosalicylanilide (TCSA), and pentachlorophenol (PCP), on transport of glycine and proline by Bacillus subtilis were examined. FCCP inhibited proline uptake uncompetitively, but glycine uptake competitively. TCSA inhibited proline uptake noncompetitively, but glycine uptake competitively. PCP inhibited proline uptake noncompetitively, but glycine uptake uncompetitively. The results indicate that these uncouplers inhibit amino acid transport by interacting at specific sites rather than by reducing any central supply of energy used to fuel metabolic processes.

Original languageEnglish (US)
Pages (from-to)368-372
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume178
Issue number2
DOIs
StatePublished - Jan 30 1977

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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