TY - JOUR
T1 - Inhibition of amino acid transport in Bacillus subtilis by uncouplers of oxidative phosphorylation
AU - Brummett, Thomas B.
AU - Ordal, George W.
N1 - ’ This investigation was supported by Grant GM 21130 from the National Institute of General Medical Sciences of the NIH. Z To whom all correspondence dressed.
PY - 1977/1/30
Y1 - 1977/1/30
N2 - The effect of three uncouplers of oxidative phosphorylation, trifluoromethoxycarbon-ylcyanidephenylhydrazone (FCCP), 3,3′,4′,5-tetrachlorosalicylanilide (TCSA), and pentachlorophenol (PCP), on transport of glycine and proline by Bacillus subtilis were examined. FCCP inhibited proline uptake uncompetitively, but glycine uptake competitively. TCSA inhibited proline uptake noncompetitively, but glycine uptake competitively. PCP inhibited proline uptake noncompetitively, but glycine uptake uncompetitively. The results indicate that these uncouplers inhibit amino acid transport by interacting at specific sites rather than by reducing any central supply of energy used to fuel metabolic processes.
AB - The effect of three uncouplers of oxidative phosphorylation, trifluoromethoxycarbon-ylcyanidephenylhydrazone (FCCP), 3,3′,4′,5-tetrachlorosalicylanilide (TCSA), and pentachlorophenol (PCP), on transport of glycine and proline by Bacillus subtilis were examined. FCCP inhibited proline uptake uncompetitively, but glycine uptake competitively. TCSA inhibited proline uptake noncompetitively, but glycine uptake competitively. PCP inhibited proline uptake noncompetitively, but glycine uptake uncompetitively. The results indicate that these uncouplers inhibit amino acid transport by interacting at specific sites rather than by reducing any central supply of energy used to fuel metabolic processes.
UR - http://www.scopus.com/inward/record.url?scp=0017369198&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017369198&partnerID=8YFLogxK
U2 - 10.1016/0003-9861(77)90205-3
DO - 10.1016/0003-9861(77)90205-3
M3 - Article
C2 - 402118
AN - SCOPUS:0017369198
SN - 0003-9861
VL - 178
SP - 368
EP - 372
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 2
ER -