Influence of salts and natural organic matter on the stability of bacteriophage MS2

Steven E. Mylon, Claudia I. Rinciog, Nathan Schmidt, Leonardo Gutierrez, Gerard C.L. Wong, Thanh H. Nguyen

Research output: Contribution to journalArticle

Abstract

The stability of functionalized nanoparticles generally results from both steric and electrostatic interactions. Viruses like bacteriophage MS2 have adopted, similar strategies for stability against aggregation, including a net negative charge under natural water conditions and using polypeptides that form loops extending from the surface of the protein capsid for stabilization. In natural systems, dissolved organic matter can adsorb to and effectively functionalize nanoparticle surfaces, affecting the fate and transport of these nanoparticles. We used time-resolved dynamic light scattering to measure the aggregation kinetics of a model virus, bacteriophage MS2, across a range of solution chemistries to determine what factors might destabilize viruses in aquatic systems. In monovalent electrolytes (LiCl, NaCl, and KCl), aggregation of MS2 could not be induced within a reasonable kinetic time frame, and MS2 was stable even at salt concentrations greater than 1.0 M. Aggregation of MS2 could be induced, in divalent electrolytes when we employed Ca2+. This trend was also observed, in solutions containing 10 mg/L Suwannee River organic matter (SROM) reference material. Even at Ca2+ concentrations as high 200 mM, diffusion-controlled aggregation was never achieved, demonstrating an additional barrier to aggregation. These results were confirmed by small-angle X-ray scattering experiments, which indicate a transition from repulsive to attractive interactions between MS2 virus particles as monovalent salts are replaced, by divalent salts.

Original languageEnglish (US)
Pages (from-to)1035-1042
Number of pages8
JournalLangmuir
Volume26
Issue number2
DOIs
StatePublished - Jan 19 2010

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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  • Cite this

    Mylon, S. E., Rinciog, C. I., Schmidt, N., Gutierrez, L., Wong, G. C. L., & Nguyen, T. H. (2010). Influence of salts and natural organic matter on the stability of bacteriophage MS2. Langmuir, 26(2), 1035-1042. https://doi.org/10.1021/la902290t