Influence of nitrogen form and NH4+-N/:NO3--N ratios on adventitious shoot formation from pear (Pyrus communis) leaf explants in vitro

Hassan Abu-Qaoud; R. M. Skirvin; F. E. Below

doi:10.1007/BF00157597

Influence of nitrogen form and NH₄⁺-N/:NO₃^--N ratios on adventitious shoot formation from pear (Pyrus communis) leaf explants in vitro

Hassan Abu-Qaoud, R. M. Skirvin, F. E. Below

Crop Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of various basal salts media, containing different nitrogen levels on in vitro adventitious shoot regeneration from leaf explants of 'Louise Bonne Panachee' and 'Seckel' pear (Pyrus communis L.) were investigated. Among the different basal salt formulae tested, Nitsch (1969) gave significantly better regeneration in most of the experiments. Shoot regeneration was altered with different NH₄⁺-N/NO₃^--N ratios. The best regeneration was obtained when NH₄⁺:NO₃^- was either 1:2 or 1:3 regardless of overall N concentration. In addition, these data show that NH₄⁺ was essential for adventitious shoot regeneration from pear leaf explants on White's (1943) medium.

Original language	English (US)
Pages (from-to)	315-319
Number of pages	5
Journal	Plant Cell, Tissue and Organ Culture
Volume	27
Issue number	3
DOIs	https://doi.org/10.1007/BF00157597
State	Published - Dec 1991

Keywords

Pyrus communis
regeneration

ASJC Scopus subject areas

Horticulture

Online availability

10.1007/BF00157597

Library availability

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Cite this

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title = "Influence of nitrogen form and NH4+-N/:NO3--N ratios on adventitious shoot formation from pear (Pyrus communis) leaf explants in vitro",

abstract = "The effect of various basal salts media, containing different nitrogen levels on in vitro adventitious shoot regeneration from leaf explants of 'Louise Bonne Panachee' and 'Seckel' pear (Pyrus communis L.) were investigated. Among the different basal salt formulae tested, Nitsch (1969) gave significantly better regeneration in most of the experiments. Shoot regeneration was altered with different NH4+-N/NO3--N ratios. The best regeneration was obtained when NH4+:NO3- was either 1:2 or 1:3 regardless of overall N concentration. In addition, these data show that NH4+ was essential for adventitious shoot regeneration from pear leaf explants on White's (1943) medium.",

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