Influence of nitrogen form and NH4+-N/:NO3--N ratios on adventitious shoot formation from pear (Pyrus communis) leaf explants in vitro

Hassan Abu-Qaoud, R. M. Skirvin, F. E. Below

Research output: Contribution to journalArticle

Abstract

The effect of various basal salts media, containing different nitrogen levels on in vitro adventitious shoot regeneration from leaf explants of 'Louise Bonne Panachee' and 'Seckel' pear (Pyrus communis L.) were investigated. Among the different basal salt formulae tested, Nitsch (1969) gave significantly better regeneration in most of the experiments. Shoot regeneration was altered with different NH4+-N/NO3--N ratios. The best regeneration was obtained when NH4+:NO3- was either 1:2 or 1:3 regardless of overall N concentration. In addition, these data show that NH4+ was essential for adventitious shoot regeneration from pear leaf explants on White's (1943) medium.

Original languageEnglish (US)
Pages (from-to)315-319
Number of pages5
JournalPlant Cell, Tissue and Organ Culture
Volume27
Issue number3
DOIs
StatePublished - Dec 1 1991

Keywords

  • Pyrus communis
  • regeneration

ASJC Scopus subject areas

  • Horticulture

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