Influence of bombardment with BA-coated microprojectiles on shoot organogenesis from Phlox paniculata L. and Pinus pinea L. tissues

I. W. Sul; S. S. Korban

doi:10.1007/BF02822738

Influence of bombardment with BA-coated microprojectiles on shoot organogenesis from Phlox paniculata L. and Pinus pinea L. tissues

I. W. Sul, S. S. Korban

Natural Resources and Environmental Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Leaves of Phlox paniculata L. were bombarded with BA-coated gold microprojectiles with the Biolistic PDS/1000-He gene gun. Increasing the concentration of BA from 0.01 to 0.1 mg/μl increased the mean number of adventitious shoots from 7 to 16 shoots per leaf. Bombardment of cotyledons of Pinus pinea L. (Italian stone pine) with BA-coated gold particles significantly reduced the frequency of shoot organogenesis by about 40%. However, induced shoot regenerants from bombarded explants of both P. paniculata and P. pinea were longer (>1 cm) than those derived from control explants after 3 and 5 wk following bombardment, respectively.

Original language	English (US)
Pages (from-to)	300-302
Number of pages	3
Journal	In Vitro Cellular and Developmental Biology - Plant
Volume	34
Issue number	4
DOIs	https://doi.org/10.1007/BF02822738
State	Published - 1998

Keywords

Cytokinin
Microprojectile bombardment
Organogenesis
Phlox paniculata
Pinus pinea

ASJC Scopus subject areas

Biotechnology
Plant Science

Online availability

10.1007/BF02822738

Library availability

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title = "Influence of bombardment with BA-coated microprojectiles on shoot organogenesis from Phlox paniculata L. and Pinus pinea L. tissues",

abstract = "Leaves of Phlox paniculata L. were bombarded with BA-coated gold microprojectiles with the Biolistic PDS/1000-He gene gun. Increasing the concentration of BA from 0.01 to 0.1 mg/μl increased the mean number of adventitious shoots from 7 to 16 shoots per leaf. Bombardment of cotyledons of Pinus pinea L. (Italian stone pine) with BA-coated gold particles significantly reduced the frequency of shoot organogenesis by about 40%. However, induced shoot regenerants from bombarded explants of both P. paniculata and P. pinea were longer (>1 cm) than those derived from control explants after 3 and 5 wk following bombardment, respectively.",

keywords = "Cytokinin, Microprojectile bombardment, Organogenesis, Phlox paniculata, Pinus pinea",

author = "Sul, {I. W.} and Korban, {S. S.}",

note = "Funding Information: This project was supported in part by the ACES Office of Research project 65-0325.",

year = "1998",

doi = "10.1007/BF02822738",

language = "English (US)",

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journal = "In Vitro Cellular and Developmental Biology - Plant",

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AU - Sul, I. W.

AU - Korban, S. S.

N1 - Funding Information: This project was supported in part by the ACES Office of Research project 65-0325.

PY - 1998

Y1 - 1998

N2 - Leaves of Phlox paniculata L. were bombarded with BA-coated gold microprojectiles with the Biolistic PDS/1000-He gene gun. Increasing the concentration of BA from 0.01 to 0.1 mg/μl increased the mean number of adventitious shoots from 7 to 16 shoots per leaf. Bombardment of cotyledons of Pinus pinea L. (Italian stone pine) with BA-coated gold particles significantly reduced the frequency of shoot organogenesis by about 40%. However, induced shoot regenerants from bombarded explants of both P. paniculata and P. pinea were longer (>1 cm) than those derived from control explants after 3 and 5 wk following bombardment, respectively.

AB - Leaves of Phlox paniculata L. were bombarded with BA-coated gold microprojectiles with the Biolistic PDS/1000-He gene gun. Increasing the concentration of BA from 0.01 to 0.1 mg/μl increased the mean number of adventitious shoots from 7 to 16 shoots per leaf. Bombardment of cotyledons of Pinus pinea L. (Italian stone pine) with BA-coated gold particles significantly reduced the frequency of shoot organogenesis by about 40%. However, induced shoot regenerants from bombarded explants of both P. paniculata and P. pinea were longer (>1 cm) than those derived from control explants after 3 and 5 wk following bombardment, respectively.

KW - Cytokinin

KW - Microprojectile bombardment

KW - Organogenesis

KW - Phlox paniculata

KW - Pinus pinea

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Influence of bombardment with BA-coated microprojectiles on shoot organogenesis from Phlox paniculata L. and Pinus pinea L. tissues

Abstract

Keywords

ASJC Scopus subject areas

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