Inflammatory Signaling via PEIZO1 Engages and Enhances the LPS-Mediated Apoptosis during Clinical Mastitis

Jingyi Wang, Ming Li, Wenda Wu, Hui Jing Zhang, Yue Yang, Muhammad Usman, Ben Aernouts, Juan J. Loor, Chuang Xu

Research output: Contribution to journalArticlepeer-review

Abstract

Bovine clinical mastitis is characterized by inflammation and immune responses, with apoptosis of mammary epithelial cells as a cellular reaction to infection. PIEZO1, identified as a mechanotransduction effector channel in nonruminant animals and sensitive to both mechanical stimuli or inflammatory signals like lipopolysaccharide (LPS). However, its role in inflammatory processes in cattle has not been well-documented. The aim of this study was to elucidate the in situ expression of PIEZO1 in bovine mammary gland and its potential involvement in clinical mastitis. We observed widespread distribution and upregulation of PIEZO1 in mammary epithelial cells in clinical mastitis cows and LPS-induced mouse models, indicating a conserved role across species. In vitro studies using mammary epithelial cells (MAC-T) revealed that LPS upregulates PIEZO1. Notably, the effects of PIEZO1 artificial activator Yoda1 increased apoptosis and NLRP3 expression, effects mitigated by PIEZO1 silencing or NLRP3 inhibition. In conclusion, the activation of the PIEZO1-NLRP3 pathway induces abnormal apoptosis in mammary epithelial cells, potentially serving as a regulatory mechanism to combat inflammatory responses to abnormal stimuli.

Original languageEnglish (US)
Pages (from-to)20321-20330
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume72
Issue number37
DOIs
StatePublished - Sep 18 2024

Keywords

  • apoptosis
  • clinical mastitis
  • PIEZO1
  • Yoda1

ASJC Scopus subject areas

  • General Chemistry
  • General Agricultural and Biological Sciences

Fingerprint

Dive into the research topics of 'Inflammatory Signaling via PEIZO1 Engages and Enhances the LPS-Mediated Apoptosis during Clinical Mastitis'. Together they form a unique fingerprint.

Cite this