Inertial microfluidic cell hydroporator (IMCH) for high-throughput & single-step intracellular molecule delivery

Yanxiang Deng, Miran Rada, Megan Kizer, Xing Wang, Dong Joo Cheon, Aram J. Chung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a high-throughput and single-step inertial microfluidic cell hydroporator (iMCH) capable of delivering diverse molecules to various cell types with a throughput of >30,000 cells/sec. Our approach is comprised of three sequential processes: (1) Cell injection, where cell suspensions with macromolecules are injected into a microchannel, (2) Inertial cell positioning, where randomly incoming cells are aligned via fluid inertia, and (3) Molecule delivery, where cells deform with large strain upon collision at a T-junction, and the rapid mechanical deformation of cells creates transient membrane openings that facilitate diffusion of molecules into the cytosol.

Original languageEnglish (US)
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages169-170
Number of pages2
ISBN (Electronic)9780692941836
StatePublished - 2020
Externally publishedYes
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: Oct 22 2017Oct 26 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period10/22/1710/26/17

Keywords

  • Inertial microfluidic cell hydroporator (iMCH)
  • Inertial microfluidics
  • Intracellular delivery

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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