Induction of somatic mutations in Tradescantia clone 4430 by three phenylenediamine isomers and the antimutagenic mechanisms of diethylditiocarbamate and ammonium meta-vanadate

Tomáš Gichner, G. Cabrera Lopez, Elizabeth D. Wagner, Michael J. Plewa

Research output: Contribution to journalArticle

Abstract

Three isomers of the promutagen phenyleldiamine at mM concentrations were plant-activated and induced mutation in stamen hairs of Tradescantia clone 4430. The rank order of the mutagenicity of the isomers was: o-phenylenediamine > m-phenyleldiamine > p-phenylenediamine with corresponding mutagenic potencies of 5.60, 1.43, and 0.46 mutant stamen hair cells/μmole, respectively. Diethyldithiocarbamate (DEDTC) and ammonium meta-vanadale (vanadate) repressed the mutagenic activity of o-phynylenediamine (o-PDA) in intact plants. Based on inhibition kinetics and reaction rates, the mechanism of DEDTC antimutagenicity was attributed to the inhibition of peroxidases that are required in the plant activation of o-PDA to mutagenic product(s). Spectrophotometric measurements of equimolar concentrations of o-PDA and vanadate demonstrated that the antimutagenic property of vanadate was mainly due to its reactivity with o-PDA.

Original languageEnglish (US)
Pages (from-to)165-172
Number of pages8
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume306
Issue number2
DOIs
StatePublished - Apr 15 1994

Keywords

  • Aromatic amines
  • Peroxidase
  • Plant activation
  • Promutagens
  • Structure-function relatiohsips

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

Fingerprint Dive into the research topics of 'Induction of somatic mutations in Tradescantia clone 4430 by three phenylenediamine isomers and the antimutagenic mechanisms of diethylditiocarbamate and ammonium meta-vanadate'. Together they form a unique fingerprint.

  • Cite this