Abstract
Three isomers of the promutagen phenyleldiamine at mM concentrations were plant-activated and induced mutation in stamen hairs of Tradescantia clone 4430. The rank order of the mutagenicity of the isomers was: o-phenylenediamine > m-phenyleldiamine > p-phenylenediamine with corresponding mutagenic potencies of 5.60, 1.43, and 0.46 mutant stamen hair cells/μmole, respectively. Diethyldithiocarbamate (DEDTC) and ammonium meta-vanadale (vanadate) repressed the mutagenic activity of o-phynylenediamine (o-PDA) in intact plants. Based on inhibition kinetics and reaction rates, the mechanism of DEDTC antimutagenicity was attributed to the inhibition of peroxidases that are required in the plant activation of o-PDA to mutagenic product(s). Spectrophotometric measurements of equimolar concentrations of o-PDA and vanadate demonstrated that the antimutagenic property of vanadate was mainly due to its reactivity with o-PDA.
Original language | English (US) |
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Pages (from-to) | 165-172 |
Number of pages | 8 |
Journal | Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis |
Volume | 306 |
Issue number | 2 |
DOIs | |
State | Published - Apr 15 1994 |
Keywords
- Aromatic amines
- Peroxidase
- Plant activation
- Promutagens
- Structure-function relatiohsips
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Health, Toxicology and Mutagenesis