Induction of somatic mutations in Tradescantia clone 4430 by three phenylenediamine isomers and the antimutagenic mechanisms of diethylditiocarbamate and ammonium meta-vanadate

Tomáš Gichner, G. Cabrera Lopez, Elizabeth D. Wagner, Michael Jacob Plewa

Research output: Contribution to journalArticle

Abstract

Three isomers of the promutagen phenyleldiamine at mM concentrations were plant-activated and induced mutation in stamen hairs of Tradescantia clone 4430. The rank order of the mutagenicity of the isomers was: o-phenylenediamine > m-phenyleldiamine > p-phenylenediamine with corresponding mutagenic potencies of 5.60, 1.43, and 0.46 mutant stamen hair cells/μmole, respectively. Diethyldithiocarbamate (DEDTC) and ammonium meta-vanadale (vanadate) repressed the mutagenic activity of o-phynylenediamine (o-PDA) in intact plants. Based on inhibition kinetics and reaction rates, the mechanism of DEDTC antimutagenicity was attributed to the inhibition of peroxidases that are required in the plant activation of o-PDA to mutagenic product(s). Spectrophotometric measurements of equimolar concentrations of o-PDA and vanadate demonstrated that the antimutagenic property of vanadate was mainly due to its reactivity with o-PDA.

Original languageEnglish (US)
Pages (from-to)165-172
Number of pages8
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume306
Issue number2
DOIs
StatePublished - Apr 15 1994

Fingerprint

Tradescantia
Phenylenediamines
Vanadates
Ammonium Compounds
Ditiocarb
Clone Cells
Mutation
Peroxidases

Keywords

  • Aromatic amines
  • Peroxidase
  • Plant activation
  • Promutagens
  • Structure-function relatiohsips

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

Cite this

Induction of somatic mutations in Tradescantia clone 4430 by three phenylenediamine isomers and the antimutagenic mechanisms of diethylditiocarbamate and ammonium meta-vanadate. / Gichner, Tomáš; Lopez, G. Cabrera; Wagner, Elizabeth D.; Plewa, Michael Jacob.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 306, No. 2, 15.04.1994, p. 165-172.

Research output: Contribution to journalArticle

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