Induction of cytochrome P450-mediated detoxification of xanthotoxin in the black swallowtail

Michael B. Cohen, May R Berenbaum, Mary A Schuler

Research output: Contribution to journalArticle

Abstract

Xanthotoxin is a phototoxic allomone found in many of the host plants of the black swallowtail, Papilio polyxenes (Lepidoptera: Papilionidae). When added to the diet of final instar larvae, xanthotoxin can induce the cytochrome P450 monooxygenase (P450) activity in midgut microsomes by which it is detoxified. Induction is dose-dependent, increasing sevenfold when larvae feed on parsley treated topically with xanthotoxin at 0.5 or 1.0% fresh weight. Although xanthotoxin exerts much of its toxic effects when photoactivated by ultraviolet light, induction of P450 activity did not differ in the presence or absence of ultraviolet light. Despite a 4.7-fold induction of xanthotoxin-metabolizing P450 activity, total P450 content measured in the same microsomal samples did not increase significantly. These data indicate that multiple forms of P450 exist in the black swallowtail midgut and that they are differentially induced by xanthotoxin.

Original languageEnglish (US)
Pages (from-to)2347-2355
Number of pages9
JournalJournal of Chemical Ecology
Volume15
Issue number9
DOIs
StatePublished - Sep 1 1989

Fingerprint

Papilio polyxenes
methoxsalen
Methoxsalen
Detoxification
detoxification
cytochrome P-450
Cytochrome P-450 Enzyme System
cytochrome
larva
host plant
Ultraviolet Rays
diet
fold
midgut
Larva
ultraviolet radiation
allomones
Petroselinum
Papilionidae
parsley

Keywords

  • Black swallowtail
  • Lepidoptera
  • Papilio polyxenes
  • Papilionidae
  • furanocoumarins
  • induction
  • microsomal cytochrome P450
  • plant defensive compounds
  • xanthotoxin

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry

Cite this

Induction of cytochrome P450-mediated detoxification of xanthotoxin in the black swallowtail. / Cohen, Michael B.; Berenbaum, May R; Schuler, Mary A.

In: Journal of Chemical Ecology, Vol. 15, No. 9, 01.09.1989, p. 2347-2355.

Research output: Contribution to journalArticle

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