TY - JOUR
T1 - Inducible Control of mRNA Transport Using Reprogrammable RNA-Binding Proteins
AU - Abil, Zhanar
AU - Gumy, Laura F.
AU - Zhao, Huimin
AU - Hoogenraad, Casper C.
N1 - We thank Dr. Hee Jung Chung and John Paul Cavaretta at the University of Illinois for kindly sharing the rat hippocampal neurons for the RNA-FISH experiments. We also thank the Core Facilities at the Carl R. Woese Institute for Genomic Biology and the Biology Imaging Center at the Utrecht University for their help with fluorescence microscopy. This work was financially supported by the Centennial Department Professorship in the Department of Chemical and Biomolecular Engineering at the University of Illinois at Urbana-Champaign (H.Z.), The Netherlands Organization for Scientific Research (NWO-ALW-VICI C.C.H.), and FP7 EU Marie Curie postdoctoral fellowship to L.F.G.
PY - 2017/6/16
Y1 - 2017/6/16
N2 - Localization of mRNA is important in a number of cellular processes such as embryogenesis, cellular motility, polarity, and a variety of neurological processes. A synthetic device that controls cellular mRNA localization would facilitate investigations on the significance of mRNA localization in cellular function and allow an additional level of controlling gene expression. In this work, we developed the PUF (Pumilio and FBF homology domain)-assisted localization of RNA (PULR) system, which utilizes a eukaryotic cell's cytoskeletal transport machinery to reposition mRNA within a cell. Depending on the cellular motor used, we show ligand-dependent transport of mRNA toward either pole of the microtubular network of cultured cells. In addition, implementation of the reprogrammable PUF domain allowed the transport of untagged endogenous mRNA in primary neurons.
AB - Localization of mRNA is important in a number of cellular processes such as embryogenesis, cellular motility, polarity, and a variety of neurological processes. A synthetic device that controls cellular mRNA localization would facilitate investigations on the significance of mRNA localization in cellular function and allow an additional level of controlling gene expression. In this work, we developed the PUF (Pumilio and FBF homology domain)-assisted localization of RNA (PULR) system, which utilizes a eukaryotic cell's cytoskeletal transport machinery to reposition mRNA within a cell. Depending on the cellular motor used, we show ligand-dependent transport of mRNA toward either pole of the microtubular network of cultured cells. In addition, implementation of the reprogrammable PUF domain allowed the transport of untagged endogenous mRNA in primary neurons.
KW - Pumilio and fem3 mRNA-binding factor (PUF)
KW - RNA-binding proteins (RBP)
KW - dynein
KW - kinesin
KW - mRNA transport
UR - https://www.scopus.com/pages/publications/85020866756
UR - https://www.scopus.com/pages/publications/85020866756#tab=citedBy
U2 - 10.1021/acssynbio.7b00025
DO - 10.1021/acssynbio.7b00025
M3 - Article
C2 - 28260376
AN - SCOPUS:85020866756
SN - 2161-5063
VL - 6
SP - 950
EP - 956
JO - ACS synthetic biology
JF - ACS synthetic biology
IS - 6
ER -