Induced coupling between contractile cells

Brian J. Williams, M. Taher A. Saif

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cells communicate with each other through biochemical and electrical mechanisms employing various autocrine and endocrine signaling pathways. Cells, and cardiomyocytes in particular, generate contractile forces on the substrates (or tissues) they adhere to. This results in a tensile strain field in the substrate around the contractile cell. As a result, nearby cells adhered to the same substrate get stretched. The effect of this long range communication has received limited attention to date. Here we develop an elastic cell culture substrate to explore strain mediated coupling in cardiomyocytes. We investigate (1) whether strain coupling of isolated cardiomyocytes affects the temporal dynamics of contractility, (2) whether such long range communication can enable synchrony in cardiomyocyte beating, and (3) what is the biophysical mechanism enabling strain induced coupling? Initial experiments show that strain coupled populations of neonatal rat cardiomyocytes can synchronize their beating with time. We hypothesize that the contraction of one cell cluster results in calcium influx in the coupled cell cluster via stretch sensitive ion channels. We utilize an integrate-and-fire oscillator model with strain induced coupling to predict the emergence of synchrony between cardiomyocytes. This finding may shed light on cardiac arrhythmias in stiffened, infarcted cardiac tissues where strain coupling may be compromised.

Original languageEnglish (US)
Title of host publicationMechanics of Biological Systems and Materials - Proceedings of the 2015 Annual Conference on Experimental and Applied Mechanics
EditorsChad S. Korach, Srinivasan Arjun Tekalur, Pablo Zavattieri
PublisherSpringer New York LLC
Pages91-97
Number of pages7
ISBN (Print)9783319214542
DOIs
StatePublished - Jan 1 2016
EventSEM Annual Conference and Exposition on Experimental and Applied Mechanics, 2015 - Costa Mesa, United States
Duration: Jun 8 2015Jun 11 2015

Publication series

NameConference Proceedings of the Society for Experimental Mechanics Series
Volume6
ISSN (Print)2191-5644
ISSN (Electronic)2191-5652

Other

OtherSEM Annual Conference and Exposition on Experimental and Applied Mechanics, 2015
CountryUnited States
CityCosta Mesa
Period6/8/156/11/15

Fingerprint

Substrates
Tissue
Tensile strain
Communication
Cell culture
Rats
Calcium
Fires
Ions
Experiments

Keywords

  • Cardiomyocytes
  • Oscillators
  • PIV
  • Strain-coupling
  • Synchronization

ASJC Scopus subject areas

  • Engineering(all)
  • Computational Mechanics
  • Mechanical Engineering

Cite this

Williams, B. J., & Saif, M. T. A. (2016). Induced coupling between contractile cells. In C. S. Korach, S. A. Tekalur, & P. Zavattieri (Eds.), Mechanics of Biological Systems and Materials - Proceedings of the 2015 Annual Conference on Experimental and Applied Mechanics (pp. 91-97). (Conference Proceedings of the Society for Experimental Mechanics Series; Vol. 6). Springer New York LLC. https://doi.org/10.1007/978-3-319-21455-9_10

Induced coupling between contractile cells. / Williams, Brian J.; Saif, M. Taher A.

Mechanics of Biological Systems and Materials - Proceedings of the 2015 Annual Conference on Experimental and Applied Mechanics. ed. / Chad S. Korach; Srinivasan Arjun Tekalur; Pablo Zavattieri. Springer New York LLC, 2016. p. 91-97 (Conference Proceedings of the Society for Experimental Mechanics Series; Vol. 6).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Williams, BJ & Saif, MTA 2016, Induced coupling between contractile cells. in CS Korach, SA Tekalur & P Zavattieri (eds), Mechanics of Biological Systems and Materials - Proceedings of the 2015 Annual Conference on Experimental and Applied Mechanics. Conference Proceedings of the Society for Experimental Mechanics Series, vol. 6, Springer New York LLC, pp. 91-97, SEM Annual Conference and Exposition on Experimental and Applied Mechanics, 2015, Costa Mesa, United States, 6/8/15. https://doi.org/10.1007/978-3-319-21455-9_10
Williams BJ, Saif MTA. Induced coupling between contractile cells. In Korach CS, Tekalur SA, Zavattieri P, editors, Mechanics of Biological Systems and Materials - Proceedings of the 2015 Annual Conference on Experimental and Applied Mechanics. Springer New York LLC. 2016. p. 91-97. (Conference Proceedings of the Society for Experimental Mechanics Series). https://doi.org/10.1007/978-3-319-21455-9_10
Williams, Brian J. ; Saif, M. Taher A. / Induced coupling between contractile cells. Mechanics of Biological Systems and Materials - Proceedings of the 2015 Annual Conference on Experimental and Applied Mechanics. editor / Chad S. Korach ; Srinivasan Arjun Tekalur ; Pablo Zavattieri. Springer New York LLC, 2016. pp. 91-97 (Conference Proceedings of the Society for Experimental Mechanics Series).
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