Incorporation of charged residues in the CYP2J2 F-G loop disrupts CYP2J2-lipid bilayer interactions

Keyphrases

• Lipid Bilayer 100%
• Charged Residue 100%
• CYP2J2 100%
• G-loop 100%
• Molecular Dynamics Simulation 20%
• Membrane Binding 20%
• Membrane Insertion 20%
• Hydrophobic Residues 20%
• Nanodiscs 10%
• Insertion Depth 10%
• Escherichia Coli 10%
• Fluorescence Spectroscopy 10%
• Enzymatic Activity 10%
• Phospholipids 10%
• Membrane Mimetics 10%
• Binding Events 10%
• Membrane Interaction 10%
• Double mutant 10%
• Fatty Acid Metabolism 10%
• Pyrene 10%
• Protein-lipid Interactions 10%
• Fluorescence Quenching 10%
• Cytochrome P450 (CYP450) 10%
• Native-like 10%
• Epoxygenase 10%
• Functional Data 10%
• Complex Proteins 10%
• Membrane Topology 10%
• Exogenous Fatty Acids 10%
• Cytosolic Fraction 10%
• Membrane Mimetic Models 10%
• POPC Membrane 10%

Biochemistry, Genetics and Molecular Biology

• Lipid Bilayer 100%
• CYP2J2 100%
• N-Terminus 10%
• Protein-Lipid Interaction 10%
• Escherichia coli 10%
• Enzyme Activity 10%
• Palmitoyl Oleoyl Phosphatidyl Choline 10%
• Fatty Acid Metabolism 10%
• Membrane Topology 10%
• Quenching (Fluorescence) 10%
• Epoxygenase 10%
• Membrane Binding 10%
• Cytosolic Fraction 10%
• Cytochrome P450 10%
• Phospholipid 10%
• Fluorescence Spectroscopy 10%