Incorporation of a fluorescent compound by live Heterodera glycines

N. E. Schroeder, A. E. MacGuidwin

Research output: Contribution to journalArticlepeer-review


The incorporation of fluorescein isothiocyanate (FITC) by J2 of Heterodera glycines, the soybean cyst nematode, and the resulting effects on fitness were determined. Live soybean cyst nematode J2 incubated in FITC fluoresced, primarily in the intestinal region, beyond auto-fluorescence. Dissection of animals, as well as fluorescence-quenching techniques, indicated that FITC was not simply bound to the cuticle. FITC was also found to cross the egg shell. Fluorescence increased in relation to FITC concentration and incubation time. Nematodes incubated in FITC remained active and did not lose their fluorescence even after two weeks at room temperature. Fluorescence of nematodes was not stable through development. Males which developed from fluorescent juveniles did not retain the stain. Both FITC and the DMF solvent reduced the hatching rate. However, those individuals that successfully hatched remained viable and able to infect roots. Incorporation of FITC was found to occur in three other genera of nematodes. Rhodamine B isothiocyanate was also found to be incorporated by H. glycines.

Original languageEnglish (US)
Pages (from-to)43-49
Number of pages7
JournalJournal of Nematology
Issue number1
StatePublished - Mar 2007
Externally publishedYes


  • Behavior
  • Chemical uptake
  • FITC
  • Fluorescein isothiocyanate
  • Heterodera glycines
  • Physiology
  • RITC
  • Technique

ASJC Scopus subject areas

  • Agronomy and Crop Science


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