TY - JOUR
T1 - Inactivation and sensitization of Pseudomonas aeruginosa by microplasma jet array for treating otitis media
AU - Sun, Peter P.
AU - Won, Jungeun
AU - Choo-Kang, Gabrielle
AU - Li, Shouyan
AU - Chen, Wenyuan
AU - Monroy, Guillermo L.
AU - Chaney, Eric J.
AU - Boppart, Stephen A.
AU - Eden, J. Gary
AU - Nguyen, Thanh H.
N1 - Funding Information:
The authors greatly appreciate the support from the National Science Foundation (NSF grant 1855609 to T.H.N. and S.A.B.) and the U.S. Air Force Office of Scientific Research under grant no. FA9550-14-1-0002, as well as support from the National Institutes of Health (NIH/NIBIB R01EB013723 and R01EB028615 to S.A.B.). The authors would also like to acknowledge support from the McGinnis Medical Innovation Fellowship program. Lastly, the authors acknowledge Alex D. Jerez Roman from the Visualization Laboratory of the Beckman Institute Imaging Technology Group for generating illustrations of the device.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Otitis media (OM), known as a middle ear infection, is the leading cause of antibiotic prescriptions for children. With wide-spread use of antibiotics in OM, resistance to antibiotics continues to decrease the efficacy of the treatment. Furthermore, as the presence of a middle ear biofilm has contributed to this reduced susceptibility to antimicrobials, effective interventions are necessary. A miniaturized 3D-printed microplasma jet array has been developed to inactivate Pseudomonas aeruginosa, a common bacterial strain associated with OM. The experiments demonstrate the disruption of planktonic and biofilm P. aeruginosa by long-lived molecular species generated by microplasma, as well as the synergy of combining microplasma treatment with antibiotic therapy. In addition, a middle ear phantom model was developed with an excised rat eardrum to investigate the antimicrobial effects of microplasma on bacteria located behind the eardrum, as in a patient-relevant setup. These results suggest the potential for microplasma as a new treatment paradigm for OM.
AB - Otitis media (OM), known as a middle ear infection, is the leading cause of antibiotic prescriptions for children. With wide-spread use of antibiotics in OM, resistance to antibiotics continues to decrease the efficacy of the treatment. Furthermore, as the presence of a middle ear biofilm has contributed to this reduced susceptibility to antimicrobials, effective interventions are necessary. A miniaturized 3D-printed microplasma jet array has been developed to inactivate Pseudomonas aeruginosa, a common bacterial strain associated with OM. The experiments demonstrate the disruption of planktonic and biofilm P. aeruginosa by long-lived molecular species generated by microplasma, as well as the synergy of combining microplasma treatment with antibiotic therapy. In addition, a middle ear phantom model was developed with an excised rat eardrum to investigate the antimicrobial effects of microplasma on bacteria located behind the eardrum, as in a patient-relevant setup. These results suggest the potential for microplasma as a new treatment paradigm for OM.
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U2 - 10.1038/s41522-021-00219-2
DO - 10.1038/s41522-021-00219-2
M3 - Article
C2 - 34078901
AN - SCOPUS:85107200268
SN - 2055-5008
VL - 7
JO - npj Biofilms and Microbiomes
JF - npj Biofilms and Microbiomes
IS - 1
M1 - 48
ER -