TY - JOUR
T1 - In vivo three-dimensional spectroscopic photoacoustic imaging for monitoring nanoparticle delivery
AU - Kim, Seungsoo
AU - Chen, Yun Sheng
AU - Luke, Geoffrey P.
AU - Emelianov, Stanislav Y.
PY - 2011/9/1
Y1 - 2011/9/1
N2 - In vivo monitoring of nanoparticle delivery is essential to better understand cellular and molecular interactions of nanoparticles with tissue and to better plan nanoparticle-mediated therapies. We developed a threedimensional ultrasound and photoacoustic (PA) imaging system and a spectroscopic PA imaging algorithm to identify and quantify the presence of nanoparticles and other tissue constituents. Using the developed system and approach, three-dimensional in vivo imaging of a mouse with tumor was performed before and after intravenous injection of gold nanorods. The developed spectroscopic PA imaging algorithm estimated distribution of nanoparticle as well as oxygen saturation of blood. Moreover, silver staining of excised tumor tissue confirmed nanoparticle deposition, and showed good correlation with spectroscopic PA images. The results of our study suggest that three-dimensional ultrasound-guided spectroscopic PA imaging can monitor nanoparticle delivery in vivo.
AB - In vivo monitoring of nanoparticle delivery is essential to better understand cellular and molecular interactions of nanoparticles with tissue and to better plan nanoparticle-mediated therapies. We developed a threedimensional ultrasound and photoacoustic (PA) imaging system and a spectroscopic PA imaging algorithm to identify and quantify the presence of nanoparticles and other tissue constituents. Using the developed system and approach, three-dimensional in vivo imaging of a mouse with tumor was performed before and after intravenous injection of gold nanorods. The developed spectroscopic PA imaging algorithm estimated distribution of nanoparticle as well as oxygen saturation of blood. Moreover, silver staining of excised tumor tissue confirmed nanoparticle deposition, and showed good correlation with spectroscopic PA images. The results of our study suggest that three-dimensional ultrasound-guided spectroscopic PA imaging can monitor nanoparticle delivery in vivo.
UR - http://www.scopus.com/inward/record.url?scp=84856159511&partnerID=8YFLogxK
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U2 - 10.1364/BOE.2.002540
DO - 10.1364/BOE.2.002540
M3 - Article
C2 - 21991546
AN - SCOPUS:84856159511
SN - 2156-7085
VL - 2
SP - 2540
EP - 2550
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 9
ER -