In vivo optical trapping indicates kinesin's stall force is reduced by dynein during intracellular transport

Benjamin H. Blehm, Trina A. Schroer, Kathleen M. Trybus, Yann R. Chemla, Paul R. Selvin

Research output: Contribution to journalArticle

Abstract

Kinesin and dynein are fundamental components of intracellular transport, but their interactions when simultaneously present on cargos are unknown. We built an optical trap that can be calibrated in vivo during data acquisition for each individual cargo to measure forces in living cells. Comparing directional stall forces in vivo and in vitro, we found evidence that cytoplasmic dynein is active during minus- and plus-end directed motion, whereas kinesin is only active in the plus direction. In vivo, we found outward (~plus-end) stall forces range from 2 to 7 pN, which is significantly less than the 5- to 7-pN stall force measured in vitro for single kinesin molecules. In vitro measurements on beads with kinesin-1 and dynein bound revealed a similar distribution, implying that an interaction between opposite polarity motors causes this difference. Finally, inward (~minus-end) stalls in vivo were 2-3 pN, which is higher than the 1.1-pN stall force of a single dynein, implying multiple active dynein.

Original languageEnglish (US)
Pages (from-to)3381-3386
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number9
DOIs
StatePublished - Feb 26 2013

Keywords

  • Microtubule
  • Molecular motors
  • Motility
  • Tug-of-war
  • Tweezers

ASJC Scopus subject areas

  • General

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