TY - JOUR
T1 - In vivo molecular and cellular imaging with quantum dots
AU - Gao, Xiaohu
AU - Yang, Lily
AU - Petros, John A.
AU - Marshall, Fray F.
AU - Simons, Jonathan W.
AU - Nie, Shuming
N1 - Funding Information:
We are grateful to Drs Andrew Smith, Gang Ruan, and Leland Chung for significant contributions to this article. We thank Lianhua Qu of CrystalPlex Corporation (Pittsburgh, PA) for help on QD synthesis. This work was supported by grants from the National Institutes of Health (grants R01 GM60562 and P20 GM072069 to SN), the Georgia Cancer Coalition (Distinguished Cancer Scholar Awards to SN and LCWC), and the Coulter Translational Research Program at Georgia Tech and Emory University. XG acknowledges the CrystalPlex Corporation for financial support and AMS thanks the Whitaker Foundation for a fellowship.
PY - 2005/2
Y1 - 2005/2
N2 - Quantum dots (QDs), tiny light-emitting particles on the nanometer scale, are emerging as a new class of fluorescent probe for in vivo biomolecular and cellular imaging. In comparison with organic dyes and fluorescent proteins, QDs have unique optical and electronic properties: size-tunable light emission, improved signal brightness, resistance against photobleaching, and simultaneous excitation of multiple fluorescence colors. Recent advances have led to the development of multifunctional nanoparticle probes that are very bright and stable under complex in vivo conditions. A new structural design involves encapsulating luminescent QDs with amphiphilic block copolymers and linking the polymer coating to tumor-targeting ligands and drug delivery functionalities. Polymer-encapsulated QDs are essentially nontoxic to cells and animals, but their long-term in vivo toxicity and degradation need more careful study. Bioconjugated QDs have raised new possibilities for ultrasensitive and multiplexed imaging of molecular targets in living cells, animal models and possibly in humans.
AB - Quantum dots (QDs), tiny light-emitting particles on the nanometer scale, are emerging as a new class of fluorescent probe for in vivo biomolecular and cellular imaging. In comparison with organic dyes and fluorescent proteins, QDs have unique optical and electronic properties: size-tunable light emission, improved signal brightness, resistance against photobleaching, and simultaneous excitation of multiple fluorescence colors. Recent advances have led to the development of multifunctional nanoparticle probes that are very bright and stable under complex in vivo conditions. A new structural design involves encapsulating luminescent QDs with amphiphilic block copolymers and linking the polymer coating to tumor-targeting ligands and drug delivery functionalities. Polymer-encapsulated QDs are essentially nontoxic to cells and animals, but their long-term in vivo toxicity and degradation need more careful study. Bioconjugated QDs have raised new possibilities for ultrasensitive and multiplexed imaging of molecular targets in living cells, animal models and possibly in humans.
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U2 - 10.1016/j.copbio.2004.11.003
DO - 10.1016/j.copbio.2004.11.003
M3 - Review article
C2 - 15722017
AN - SCOPUS:13844296384
SN - 0958-1669
VL - 16
SP - 63
EP - 72
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
IS - 1 SPEC. ISS.
ER -