In vivo incorporation of unnatural amino acids into ion channels in Xenopus oocyte expression system

Mark W. Nowak, Justin P. Gallivan, Scott K Silverman, Cesar G. Labarca, Dennis A. Dougherty, Henry A. Lester

Research output: Contribution to journalArticle

Abstract

A general method for the incorporation of unnatural amino acids into ion channels and membrane receptors using a Xenopus oocyte expression system has been described. A large number of unnatural amino acids have been incorporated into the nAChR, GIRK, and Shaker K+ channels. Continuing efforts focus on incorporating unnatural amino acids that differ substantially from the natural amino acids, for example, residues that include fluorophores. In addition, we are addressing the feasibility of incorporating unnatural amino acids into ion channels and membrane receptors in mammalian cells.

Original languageEnglish (US)
Pages (from-to)504-529
Number of pages26
JournalMethods in enzymology
Volume293
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

Fingerprint

Xenopus
Ion Channels
Oocytes
Amino Acids
Membranes
Fluorophores
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

In vivo incorporation of unnatural amino acids into ion channels in Xenopus oocyte expression system. / Nowak, Mark W.; Gallivan, Justin P.; Silverman, Scott K; Labarca, Cesar G.; Dougherty, Dennis A.; Lester, Henry A.

In: Methods in enzymology, Vol. 293, 01.01.1998, p. 504-529.

Research output: Contribution to journalArticle

Nowak, Mark W. ; Gallivan, Justin P. ; Silverman, Scott K ; Labarca, Cesar G. ; Dougherty, Dennis A. ; Lester, Henry A. / In vivo incorporation of unnatural amino acids into ion channels in Xenopus oocyte expression system. In: Methods in enzymology. 1998 ; Vol. 293. pp. 504-529.
@article{9481cdbf33e9450da507838f7e85f0a3,
title = "In vivo incorporation of unnatural amino acids into ion channels in Xenopus oocyte expression system",
abstract = "A general method for the incorporation of unnatural amino acids into ion channels and membrane receptors using a Xenopus oocyte expression system has been described. A large number of unnatural amino acids have been incorporated into the nAChR, GIRK, and Shaker K+ channels. Continuing efforts focus on incorporating unnatural amino acids that differ substantially from the natural amino acids, for example, residues that include fluorophores. In addition, we are addressing the feasibility of incorporating unnatural amino acids into ion channels and membrane receptors in mammalian cells.",
author = "Nowak, {Mark W.} and Gallivan, {Justin P.} and Silverman, {Scott K} and Labarca, {Cesar G.} and Dougherty, {Dennis A.} and Lester, {Henry A.}",
year = "1998",
month = "1",
day = "1",
doi = "10.1016/S0076-6879(98)93031-2",
language = "English (US)",
volume = "293",
pages = "504--529",
journal = "Methods in Enzymology",
issn = "0076-6879",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - In vivo incorporation of unnatural amino acids into ion channels in Xenopus oocyte expression system

AU - Nowak, Mark W.

AU - Gallivan, Justin P.

AU - Silverman, Scott K

AU - Labarca, Cesar G.

AU - Dougherty, Dennis A.

AU - Lester, Henry A.

PY - 1998/1/1

Y1 - 1998/1/1

N2 - A general method for the incorporation of unnatural amino acids into ion channels and membrane receptors using a Xenopus oocyte expression system has been described. A large number of unnatural amino acids have been incorporated into the nAChR, GIRK, and Shaker K+ channels. Continuing efforts focus on incorporating unnatural amino acids that differ substantially from the natural amino acids, for example, residues that include fluorophores. In addition, we are addressing the feasibility of incorporating unnatural amino acids into ion channels and membrane receptors in mammalian cells.

AB - A general method for the incorporation of unnatural amino acids into ion channels and membrane receptors using a Xenopus oocyte expression system has been described. A large number of unnatural amino acids have been incorporated into the nAChR, GIRK, and Shaker K+ channels. Continuing efforts focus on incorporating unnatural amino acids that differ substantially from the natural amino acids, for example, residues that include fluorophores. In addition, we are addressing the feasibility of incorporating unnatural amino acids into ion channels and membrane receptors in mammalian cells.

UR - http://www.scopus.com/inward/record.url?scp=0032311418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032311418&partnerID=8YFLogxK

U2 - 10.1016/S0076-6879(98)93031-2

DO - 10.1016/S0076-6879(98)93031-2

M3 - Article

C2 - 9711626

AN - SCOPUS:0032311418

VL - 293

SP - 504

EP - 529

JO - Methods in Enzymology

JF - Methods in Enzymology

SN - 0076-6879

ER -