In vivo incorporation of unnatural amino acids into ion channels in Xenopus oocyte expression system

Mark W. Nowak, Justin P. Gallivan, Scott K Silverman, Cesar G. Labarca, Dennis A. Dougherty, Henry A. Lester

Research output: Contribution to journalArticle

Abstract

A general method for the incorporation of unnatural amino acids into ion channels and membrane receptors using a Xenopus oocyte expression system has been described. A large number of unnatural amino acids have been incorporated into the nAChR, GIRK, and Shaker K+ channels. Continuing efforts focus on incorporating unnatural amino acids that differ substantially from the natural amino acids, for example, residues that include fluorophores. In addition, we are addressing the feasibility of incorporating unnatural amino acids into ion channels and membrane receptors in mammalian cells.

Original languageEnglish (US)
Pages (from-to)504-529
Number of pages26
JournalMethods in enzymology
Volume293
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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