In vivo enzymatic protein biotinylation

Anne Chapman-Smith, John E. Cronan

Research output: Contribution to journalArticlepeer-review


Biotin is biologically active only when protein-bound and is covalently attached to a class of important metabolic enzymes, the biotin carboxylases and decarboxylases. Biotinylation is a relatively rare modification, with between one and five biotinylated protein species found in different organisms. We discuss the mechanism and structures involved in this extraordinarily specific protein modification and its exploitation in tagging recombinant proteins. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)119-125
Number of pages7
JournalBiomolecular Engineering
Issue number1-4
StatePublished - Dec 31 1999


  • Biotin
  • Biotin ligase
  • Biotinylation
  • Carboxylases and decarboxylases
  • Protein modification

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Molecular Biology


Dive into the research topics of 'In vivo enzymatic protein biotinylation'. Together they form a unique fingerprint.

Cite this