In Vivo Confocal Imaging of Fluorescently Labeled Microbubbles: Implications for Ultrasound Localization Microscopy

Matthew R. Lowerison, Chengwu Huang, Yohan Kim, Fabrice Lucien, Shigao Chen, Pengfei Song

Research output: Contribution to journalArticlepeer-review

Abstract

We report the time kinetics of fluorescently labeled microbubbles (MBs) in capillary-level microvasculature as measured via confocal microscopy and compare these results to ultrasound localization microscopy (ULM). The observed 19.4 ± 4.2 MBs per confocal field-of-view (212μ m× 212 μ m) are in excellent agreement with the expected count of 19.1 MBs per frame. The estimated time to fully perfuse this capillary network was 193 s, which corroborates the values reported in the literature. We then modeled the capillary network as an empirically determined discrete-time Markov chain with adjustable MB transition probabilities though individual capillaries. The Monte Carlo random walk simulations found perfusion times ranging from 24.5 s for unbiased Markov chains up to 182 s for heterogeneous flow distributions. This pilot study confirms a probability-derived explanation for the long acquisition times required for super-resolution ULM.

Original languageEnglish (US)
Article number9068283
Pages (from-to)1811-1819
Number of pages9
JournalIEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
Volume67
Issue number9
DOIs
StatePublished - Sep 2020

Keywords

  • Microbubble (MB) tracking
  • microvasculature
  • super-resolution
  • ultrasound microvessel imaging

ASJC Scopus subject areas

  • Instrumentation
  • Acoustics and Ultrasonics
  • Electrical and Electronic Engineering

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