TY - JOUR
T1 - In vivo and in vitro applications of collagen-GAG scaffolds
AU - Harley, Brendan A.C.
AU - Gibson, Lorna J.
N1 - Funding Information:
The financial contributions of the Cambridge-MIT Institute (BAH, LJG), the Whitaker-MIT Health Science Fund Fellowship (BAH), NIH Grant DE 13053 (BAH), and the Matoula S. Salapatas Professorship at MIT (LJG) are gratefully recognized. The authors would like to acknowledge Dr. Andrew Lynn (OrthoMimetics, Inc., UK), Dr. Toby Freyman (MIT, now at Boston Scientific Inc., USA), Prof. Fergal O’Brien (Trinity College, Ireland), Professor Bill Bonfield (University of Cambridge, UK), Mary Waller (Trinity College), as well as Professor Merton Flemings, Professor Krystyn Van Vliet, Alan Schwartzman, Emilio Silva, the NanoMechanical Technology Laboratory, Kristin Myers, Matthew Wong, Professor Simona Socrate, and Professor Ioannis Yannas at MIT for the facilities and assistance that aided in completing this research.
PY - 2008/3/15
Y1 - 2008/3/15
N2 - Tissue engineering scaffolds are used extensively as three-dimensional analogs of the extracellular matrix (ECM). Collagen-glycosaminoglycan (CG) scaffolds have long been utilized as ECM analogs for the regeneration of skin and are currently being considered for the regeneration of nerve, conjunctiva, and a host or orthopedic tissues. Recently a series of CG scaffolds with a uniform pore microstructure has been developed with a range of sizes of equiaxed pores. Experimental characterization and theoretical modeling techniques have been used to describe the pore microstructure, specific surface area, tensile and compressive mechanical properties, cell attachment, and permeability of these variants. Here we describe the fabrication, and characterization, and modeling of a series of CG and mineralized CG scaffolds. We then discuss their use in vivo to induce tissue regeneration following injury and in vitro as standardized 3D materials to study the influence of microstructural and mechanical features on cell behaviors such as motility and contraction.
AB - Tissue engineering scaffolds are used extensively as three-dimensional analogs of the extracellular matrix (ECM). Collagen-glycosaminoglycan (CG) scaffolds have long been utilized as ECM analogs for the regeneration of skin and are currently being considered for the regeneration of nerve, conjunctiva, and a host or orthopedic tissues. Recently a series of CG scaffolds with a uniform pore microstructure has been developed with a range of sizes of equiaxed pores. Experimental characterization and theoretical modeling techniques have been used to describe the pore microstructure, specific surface area, tensile and compressive mechanical properties, cell attachment, and permeability of these variants. Here we describe the fabrication, and characterization, and modeling of a series of CG and mineralized CG scaffolds. We then discuss their use in vivo to induce tissue regeneration following injury and in vitro as standardized 3D materials to study the influence of microstructural and mechanical features on cell behaviors such as motility and contraction.
KW - Cellular solids
KW - Collagen
KW - Scaffolds
KW - Tissue engineering
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U2 - 10.1016/j.cej.2007.09.009
DO - 10.1016/j.cej.2007.09.009
M3 - Article
AN - SCOPUS:38949168463
SN - 1385-8947
VL - 137
SP - 102
EP - 121
JO - Chemical Engineering Journal
JF - Chemical Engineering Journal
IS - 1
ER -