TY - JOUR
T1 - In vitro reconstruction of the mitochondrial translation system of yeast
AU - Pfisterer, J.
AU - Buetow, D. E.
PY - 1981
Y1 - 1981
N2 - We have isolated the translation system from yeast mitochondria and have reconstructed it in vitro. This submitochrondrial system, composed of mitochondrial ribosomes, tRNA, pH 5 fraction and mRNA,is maximally active at 10 mM Mg2+ and 100 mM KCl or NH4Cl. NH4+ is more stimulatory than K+. Added Escherichia coli tRNA gives less than half the activity obtained with added mitochondrial tRNA. Activity is enhanced with protease inhibitors but not with Ca2+, spermine, or spermidine. In contrast to heterologous translation systems, the present system produces products with molecular weights similar to those of products synthesized by yeast mitochondria in vivo and by intact yeast mitochondria in vitro. The results support the idea that the unique coding features of the mitochondiral genome require a unique translation system for accurate translation of mitochondrial mRNAs.
AB - We have isolated the translation system from yeast mitochondria and have reconstructed it in vitro. This submitochrondrial system, composed of mitochondrial ribosomes, tRNA, pH 5 fraction and mRNA,is maximally active at 10 mM Mg2+ and 100 mM KCl or NH4Cl. NH4+ is more stimulatory than K+. Added Escherichia coli tRNA gives less than half the activity obtained with added mitochondrial tRNA. Activity is enhanced with protease inhibitors but not with Ca2+, spermine, or spermidine. In contrast to heterologous translation systems, the present system produces products with molecular weights similar to those of products synthesized by yeast mitochondria in vivo and by intact yeast mitochondria in vitro. The results support the idea that the unique coding features of the mitochondiral genome require a unique translation system for accurate translation of mitochondrial mRNAs.
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U2 - 10.1073/pnas.78.8.4917
DO - 10.1073/pnas.78.8.4917
M3 - Article
C2 - 6946437
AN - SCOPUS:0019786388
SN - 0027-8424
VL - 78
SP - 4917
EP - 4921
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 8 I
ER -