In vitro mineral nutrition of Curcuma longa L. Affects production of volatile compounds in rhizomes after transfer to the greenhouse

Rabia F. El-Hawaz, Mary H. Grace, Alan Janbey, Mary Ann Lila, Jeffrey W. Adelberg

Research output: Contribution to journalArticle

Abstract

Background: Turmeric is a rich source of bioactive compounds useful in both medicine and cuisine. Mineral concentrations effects (PO4 3-, Ca2+, Mg2+, and KNO3) were tested during in vitro rhizome development on the ex vitro content of volatile constituents in rhizomes after 6 months in the greenhouse. A response surface method (D-optimal criteria) was repeated in both high and low-input fertilizer treatments. Control plants were grown on Murashige and Skoog (MS) medium, acclimatized in the greenhouse and grown in the field. The volatile constituents were investigated by GC-MS. Results: The total content of volatiles was affected by fertilizer treatments, and in vitro treatment with Ca2+ and KNO3; but PO4 3- and Mg2+ had no significant effect. The content was higher in the high-input fertilizer treatments (49.7 ± 9 mg/g DM) with 4 mM Ca2+, 60 mM KNO3 and 5 mM NH4 +, than the low-input fertilizer (26.6 ± 9 mg/g DM), and the MS control (15.28 ± 2.7 mg/g DM; 3 mM Ca2+, 20 mM K+, 39 mM NO3 -, 20 mM NH4 +, 1.25 mM PO4 3-, and 1.5 mM Mg2+). The interaction of Ca2+ with KNO3 affected curcumenol isomer I and II, germacrone, isocurcumenol, and β-elemenone content. Increasing in vitro phosphate concentration to 6.25 mM increased ex vitro neocurdione and methenolone contents. Conclusion: These results show that minerals in the in vitro bioreactor medium during rhizome development affected biosynthesis of turmeric volatile components after transfer to the greenhouse six months later. The multi-factor design identified 1) nutrient regulation of specific components within unique phytochemical profile for Curcuma longa L. clone 35-1 and 2) the varied phytochemical profiles were maintained with integrity during the greenhouse growth in high fertility conditions.

Original languageEnglish (US)
Article number122
JournalBMC Plant Biology
Volume18
Issue number1
DOIs
StatePublished - Jun 18 2018

Keywords

  • Bioreactor
  • Curcuma longa rhizome
  • Fed-batch technique
  • GC-MS
  • Response surface methods
  • Sesquiterpenes

ASJC Scopus subject areas

  • Plant Science

Fingerprint Dive into the research topics of 'In vitro mineral nutrition of Curcuma longa L. Affects production of volatile compounds in rhizomes after transfer to the greenhouse'. Together they form a unique fingerprint.

  • Cite this