In vitro increase of mean corpuscular volume difference (dMCV) as a marker for serum hypertonicity in dogs

Jennifer M. Reinhart; Misty R. Yancey; Lisa M. Pohlman; Thomas Schermerhorn

doi:10.1016/j.rvsc.2014.03.005

In vitro increase of mean corpuscular volume difference (dMCV) as a marker for serum hypertonicity in dogs

Jennifer M. Reinhart, Misty R. Yancey, Lisa M. Pohlman, Thomas Schermerhorn

Research output: Contribution to journal › Article › peer-review

Abstract

Spurious increase in erythrocyte mean corpuscular volume (MCV) on automated cell analyzers is a well-characterized lab error in hypertonic patients. A difference between automated and manual MCV (dMCV) greater than 2 fl has been shown to predict hypertonicity in humans. The purpose of this study was to investigate dMCV as a marker for serum hypertonicity in dogs and to examine the relationship between dMCV and three methods of estimating serum tonicity: measured (OsM_M), calculated (OsM_C), and calculated effective (OsM_CE) osmolalities. OsM_C, OsM_CE, and dMCV were calculated from routine blood values and OsM_M was directly measured in 121 dogs. The dMCV of hypertonic dogs was significantly larger than that of normotonic dogs for all three osmolality methods. dMCV predicted hypertonicity as estimated by OsM_M better than it predicted hypertonicity as estimated by OsM_C and OsM_CE. A cutoff of 2.96 fl yielded the best sensitivity (76%) and specificity (71%) for hypertonicity estimated by OsM_M.

Original language	English (US)
Pages (from-to)	442-446
Number of pages	5
Journal	Research in Veterinary Science
Volume	96
Issue number	3
DOIs	https://doi.org/10.1016/j.rvsc.2014.03.005
State	Published - Jun 2014
Externally published	Yes

Keywords

Endocrinology
Metabolism
Osmolality
Sodium
Tonicity

ASJC Scopus subject areas

General Veterinary

Online availability

10.1016/j.rvsc.2014.03.005

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title = "In vitro increase of mean corpuscular volume difference (dMCV) as a marker for serum hypertonicity in dogs",

abstract = "Spurious increase in erythrocyte mean corpuscular volume (MCV) on automated cell analyzers is a well-characterized lab error in hypertonic patients. A difference between automated and manual MCV (dMCV) greater than 2 fl has been shown to predict hypertonicity in humans. The purpose of this study was to investigate dMCV as a marker for serum hypertonicity in dogs and to examine the relationship between dMCV and three methods of estimating serum tonicity: measured (OsMM), calculated (OsMC), and calculated effective (OsMCE) osmolalities. OsMC, OsMCE, and dMCV were calculated from routine blood values and OsMM was directly measured in 121 dogs. The dMCV of hypertonic dogs was significantly larger than that of normotonic dogs for all three osmolality methods. dMCV predicted hypertonicity as estimated by OsMM better than it predicted hypertonicity as estimated by OsMC and OsMCE. A cutoff of 2.96 fl yielded the best sensitivity (76%) and specificity (71%) for hypertonicity estimated by OsMM.",

keywords = "Endocrinology, Metabolism, Osmolality, Sodium, Tonicity",

author = "Reinhart, {Jennifer M.} and Yancey, {Misty R.} and Pohlman, {Lisa M.} and Thomas Schermerhorn",

note = "Funding Information: This work was supported in part by the Kansas State University Mark Derrick Canine Research Fund (intramural grant).",

year = "2014",

month = jun,

doi = "10.1016/j.rvsc.2014.03.005",

language = "English (US)",

volume = "96",

pages = "442--446",

journal = "Research in Veterinary Science",

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}

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T1 - In vitro increase of mean corpuscular volume difference (dMCV) as a marker for serum hypertonicity in dogs

AU - Reinhart, Jennifer M.

AU - Yancey, Misty R.

AU - Pohlman, Lisa M.

AU - Schermerhorn, Thomas

N1 - Funding Information: This work was supported in part by the Kansas State University Mark Derrick Canine Research Fund (intramural grant).

PY - 2014/6

Y1 - 2014/6

N2 - Spurious increase in erythrocyte mean corpuscular volume (MCV) on automated cell analyzers is a well-characterized lab error in hypertonic patients. A difference between automated and manual MCV (dMCV) greater than 2 fl has been shown to predict hypertonicity in humans. The purpose of this study was to investigate dMCV as a marker for serum hypertonicity in dogs and to examine the relationship between dMCV and three methods of estimating serum tonicity: measured (OsMM), calculated (OsMC), and calculated effective (OsMCE) osmolalities. OsMC, OsMCE, and dMCV were calculated from routine blood values and OsMM was directly measured in 121 dogs. The dMCV of hypertonic dogs was significantly larger than that of normotonic dogs for all three osmolality methods. dMCV predicted hypertonicity as estimated by OsMM better than it predicted hypertonicity as estimated by OsMC and OsMCE. A cutoff of 2.96 fl yielded the best sensitivity (76%) and specificity (71%) for hypertonicity estimated by OsMM.

AB - Spurious increase in erythrocyte mean corpuscular volume (MCV) on automated cell analyzers is a well-characterized lab error in hypertonic patients. A difference between automated and manual MCV (dMCV) greater than 2 fl has been shown to predict hypertonicity in humans. The purpose of this study was to investigate dMCV as a marker for serum hypertonicity in dogs and to examine the relationship between dMCV and three methods of estimating serum tonicity: measured (OsMM), calculated (OsMC), and calculated effective (OsMCE) osmolalities. OsMC, OsMCE, and dMCV were calculated from routine blood values and OsMM was directly measured in 121 dogs. The dMCV of hypertonic dogs was significantly larger than that of normotonic dogs for all three osmolality methods. dMCV predicted hypertonicity as estimated by OsMM better than it predicted hypertonicity as estimated by OsMC and OsMCE. A cutoff of 2.96 fl yielded the best sensitivity (76%) and specificity (71%) for hypertonicity estimated by OsMM.

KW - Endocrinology

KW - Metabolism

KW - Osmolality

KW - Sodium

KW - Tonicity

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In vitro increase of mean corpuscular volume difference (dMCV) as a marker for serum hypertonicity in dogs

Abstract

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