TY - JOUR
T1 - In Vitro Fermentation of Xylooligosaccharides Produced from Miscanthus × giganteus by Human Fecal Microbiota
AU - Chen, Ming Hsu
AU - Swanson, Kelly S
AU - Fahey, George C.
AU - Dien, Bruce S.
AU - Beloshapka, Alison N.
AU - Bauer, Laura L.
AU - Rausch, Kent D
AU - Tumbleson, Mike E
AU - Singh, Vijay
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2016/1/13
Y1 - 2016/1/13
N2 - Purified xylooligosaccharides from Miscanthus × giganteus (M×G XOS) were used in an in vitro fermentation experiment inoculated with human fecal microbiota. A commercial XOS product and pectin were used as controls. Decreases in pH by 2.3, 2.4, and 2.0 units and production of short-chain fatty acids (SCFA; acetic acid, 7764.2, 6664.1, and 6387.9 μmol/g; propionic acid, 1006.7, 1089.5, and 661.5 μmol/g; and butyric acid, 955.5, 1252.9, and 917.7 μmol/g) were observed in M×G XOS, commercial XOS, and pectin medium after 12 h of fermentation, respectively. Titers of Bifidobacterium spp., Lactobacillus spp., and Escherichia coli increased when fed all three substrates as monitored by qPCR. There was no significant trend for Clostridium perfringens. During fermentation, M×G XOS was statistically equivalent in performance to the commercial XOS sample as measured by culture acidification and growth of health-promoting bacteria and resulted in the highest SCFA production among the three substrates.
AB - Purified xylooligosaccharides from Miscanthus × giganteus (M×G XOS) were used in an in vitro fermentation experiment inoculated with human fecal microbiota. A commercial XOS product and pectin were used as controls. Decreases in pH by 2.3, 2.4, and 2.0 units and production of short-chain fatty acids (SCFA; acetic acid, 7764.2, 6664.1, and 6387.9 μmol/g; propionic acid, 1006.7, 1089.5, and 661.5 μmol/g; and butyric acid, 955.5, 1252.9, and 917.7 μmol/g) were observed in M×G XOS, commercial XOS, and pectin medium after 12 h of fermentation, respectively. Titers of Bifidobacterium spp., Lactobacillus spp., and Escherichia coli increased when fed all three substrates as monitored by qPCR. There was no significant trend for Clostridium perfringens. During fermentation, M×G XOS was statistically equivalent in performance to the commercial XOS sample as measured by culture acidification and growth of health-promoting bacteria and resulted in the highest SCFA production among the three substrates.
KW - Miscanthus × giganteus
KW - biorefinery
KW - coproducts
KW - fecal microbiota
KW - in vitro fermentation
KW - prebiotics
KW - xylooligosaccharides
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U2 - 10.1021/acs.jafc.5b04618
DO - 10.1021/acs.jafc.5b04618
M3 - Article
C2 - 26648520
AN - SCOPUS:84954309438
SN - 0021-8561
VL - 64
SP - 262
EP - 267
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 1
ER -