Abstract
The direct‐acting mutagen 4‐nitro‐o‐phenylenediamine (NOP) was activated in vitro by pea or tobacco S‐9 into a more potent mutagen in Salmonella typhimurium strain TA98. NOP mutagenicity was not altered by Aroclor 1254‐induced rat liver S‐9. The plant S‐9 activation of NOP was heat‐sensitive but was not NADPH‐dependent, did not involve superoxide radicals, and was not inhibited by CO. A direct relationship between plant peroxidase and NOP activation was established. Several purified peroxidases including horseradish peroxidase, chloroperoxidase, and lactoperoxidase also activated NOP. The perodoxidative process was not H2O2‐dependent but was partially inhibited by a peroxidase inhibitor.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 73-85 |
| Number of pages | 13 |
| Journal | Environmental Mutagenesis |
| Volume | 7 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1985 |
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Keywords
- 4‐nitro‐o‐phenylenediamine
- Salmonella
- in vitro
- peroxidase
- plant activation
ASJC Scopus subject areas
- Genetics
- Health, Toxicology and Mutagenesis
Cite this
In vitro enhancement of the mutagenicity of 4‐nitro‐o‐phenylenediamine by plant S‐9. / Gentile, James M.; Gentile, Glenda J.; Townsend, Steven; Plewa, Michael Jacob.
In: Environmental Mutagenesis, Vol. 7, No. 1, 1985, p. 73-85.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro enhancement of the mutagenicity of 4‐nitro‐o‐phenylenediamine by plant S‐9
AU - Gentile, James M.
AU - Gentile, Glenda J.
AU - Townsend, Steven
AU - Plewa, Michael Jacob
PY - 1985
Y1 - 1985
N2 - The direct‐acting mutagen 4‐nitro‐o‐phenylenediamine (NOP) was activated in vitro by pea or tobacco S‐9 into a more potent mutagen in Salmonella typhimurium strain TA98. NOP mutagenicity was not altered by Aroclor 1254‐induced rat liver S‐9. The plant S‐9 activation of NOP was heat‐sensitive but was not NADPH‐dependent, did not involve superoxide radicals, and was not inhibited by CO. A direct relationship between plant peroxidase and NOP activation was established. Several purified peroxidases including horseradish peroxidase, chloroperoxidase, and lactoperoxidase also activated NOP. The perodoxidative process was not H2O2‐dependent but was partially inhibited by a peroxidase inhibitor.
AB - The direct‐acting mutagen 4‐nitro‐o‐phenylenediamine (NOP) was activated in vitro by pea or tobacco S‐9 into a more potent mutagen in Salmonella typhimurium strain TA98. NOP mutagenicity was not altered by Aroclor 1254‐induced rat liver S‐9. The plant S‐9 activation of NOP was heat‐sensitive but was not NADPH‐dependent, did not involve superoxide radicals, and was not inhibited by CO. A direct relationship between plant peroxidase and NOP activation was established. Several purified peroxidases including horseradish peroxidase, chloroperoxidase, and lactoperoxidase also activated NOP. The perodoxidative process was not H2O2‐dependent but was partially inhibited by a peroxidase inhibitor.
KW - 4‐nitro‐o‐phenylenediamine
KW - Salmonella
KW - in vitro
KW - peroxidase
KW - plant activation
UR - http://www.scopus.com/inward/record.url?scp=0022415315&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022415315&partnerID=8YFLogxK
U2 - 10.1002/em.2860070104
DO - 10.1002/em.2860070104
M3 - Article
C2 - 3881253
AN - SCOPUS:0022415315
VL - 7
SP - 73
EP - 85
JO - Environmental and Molecular Mutagenesis
JF - Environmental and Molecular Mutagenesis
SN - 0893-6692
IS - 1
ER -