In vitro chemosensitivity of feline injection site-associated sarcoma cell lines to carboplatin

Elizabeth A. Maxwell, Heidi Phillips, David J. Schaeffer, Timothy M. Fan

Research output: Contribution to journalArticle

Abstract

Objective: To determine the in vitro chemosensitivity of feline injection site-associated sarcoma (FISAS) cells to carboplatin concentrations generated by elution of carboplatin-impregnated calcium sulfate hemihydrate (CI-CSH) beads. Study design: In vitro study. Sample: Five immortalized cell lines from histologically confirmed, primary FISASs. Methods: For each cell line, one 96-well microplate was used for each time point (24, 48, 72 hours). In each microplate, 3 wells were seeded with ∼7.5 × 103 cells per well for every carboplatin treatment added, ranging from 5 to 450 µM. Microculture plates were incubated for 24, 48, or 72 hours. Drug efficacy was assessed via a bioreductive fluorometric assay. For apoptosis analysis, 3 wells were seeded with ∼5 × 104 cells per well for every carboplatin treatment added, ranging from 5 to 450 µM. Flow cytometry was performed and the relative percentages of viable, apoptotic, and late apoptotic/necrotic cells were reported. All experiments were run in triplicates. Results: Carboplatin exerted dose-dependent and time-dependent effects on FISAS cell viability. The IC50 values were within the range of carboplatin concentrations eluted from CI-CSH beads. Conclusion: Elution of carboplatin from CI-CSH beads generate concentrations sufficient to result in 50% growth inhibition of FISAS cells in vitro. Local tumor control might be achieved by implantation of CI-CSH beads immediately following radical or marginal excision of the primary tumor or by implantation without tumor resection.

Original languageEnglish (US)
Pages (from-to)219-226
Number of pages8
JournalVeterinary Surgery
Volume47
Issue number2
DOIs
StatePublished - Feb 1 2018

ASJC Scopus subject areas

  • veterinary(all)

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