In vitro assay for measuring receptor-kinase activity in the bacillus subtilis chemotaxis pathway

Research output: Chapter in Book/Report/Conference proceedingChapter


The sensing apparatus of the Bacillus subtilis chemotaxis pathway involves a complex consisting of chemoreceptors, the CheA histidine kinase, and the CheV and CheW adaptor proteins. Attractants and repellents alter the rate of CheA autophosphorylation, either by directly binding the receptors or by indirectly interacting with them through intermediate binding proteins. We describe an in vitro assay for measuring receptor-kinase activity in B. subtilis. This assay has been used to investigate the mechanism of signal transduction in B. subtilis chemotaxis and the disparate mechanisms employed by this bacterium for sensory adaptation and gradient sensing.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages11
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745



  • Bacillus subtilis
  • CheA
  • Chemoreceptors
  • Chemotaxis
  • Kinase assay

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this