In vitro assay for measuring receptor-kinase activity in the bacillus subtilis chemotaxis pathway

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The sensing apparatus of the Bacillus subtilis chemotaxis pathway involves a complex consisting of chemoreceptors, the CheA histidine kinase, and the CheV and CheW adaptor proteins. Attractants and repellents alter the rate of CheA autophosphorylation, either by directly binding the receptors or by indirectly interacting with them through intermediate binding proteins. We describe an in vitro assay for measuring receptor-kinase activity in B. subtilis. This assay has been used to investigate the mechanism of signal transduction in B. subtilis chemotaxis and the disparate mechanisms employed by this bacterium for sensory adaptation and gradient sensing.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages95-105
Number of pages11
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1729
ISSN (Print)1064-3745

Fingerprint

Chemotaxis
Bacillus subtilis
Phosphotransferases
Signal Transduction
Carrier Proteins
Bacteria
In Vitro Techniques
Proteins

Keywords

  • Bacillus subtilis
  • CheA
  • Chemoreceptors
  • Chemotaxis
  • Kinase assay

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

In vitro assay for measuring receptor-kinase activity in the bacillus subtilis chemotaxis pathway. / Walukiewicz, Hanna E.; Ordal, George W.; Rao, Christopher V.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 95-105 (Methods in Molecular Biology; Vol. 1729).

Research output: Chapter in Book/Report/Conference proceedingChapter

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